Jagged1 Recombinant Rabbit Monoclonal Antibody [PSH28-36]
Catalog# HA724777
Jagged1 Recombinant Rabbit Monoclonal Antibody [PSH28-36]
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
Jagged1 Recombinant Rabbit Monoclonal Antibody [PSH28-36]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Jagged1 aa 1,057-1,218.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 134 kDa
Positive Control
Hep G2 (Human hepatocellular carcinoma cell) cell lysate, NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate, HUVEC (Human umbilical vein endothelial cell) cell lysate, Hepa1-6 (Mouse liver cancer cell) cell lysate, bEnd.3 (Mouse brain microvascular endothelial cell) cell lysate.
Conjugation
unconjugated
Clone Number
PSH28-36
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC(Intra) | IF-Cell | IP | |
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| mouse |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
Target
Function
The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. Ligands for Notch include Jagged1, Jagged2 and Delta. Jagged is a membrane protein and can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis. Jagged is expressed in adult and fetal tissues, and expression is upregulated in cervical squamous cell carcinoma. Familial Tetralogy of Fallot, the most common form of complex congenital heart disease, is caused by a mutation in the Jagged1 gene.
Background References
1. Kim KH et al. CCN1 induces hepatic ductular reaction through integrin av 5-mediated activation of NF- B. J Clin Invest 125:1886-900 (2015).
2. Zhang X et al. FADD regulates thymocyte development at the -selection checkpoint by modulating Notch signaling. Cell Death Dis 5:e1273 (2014).
Subcellular Location
Membrane.
Synonyms
AGS antibody
AHD antibody
AWS antibody
CD 339 antibody
CD339 antibody
CD339 antigen antibody
Headturner antibody
hJ1 antibody
Htu antibody
Jag 1 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Jagged1 on different lysates with Rabbit anti-Jagged1 antibody (HA724777) at 1/2,000 dilution.
Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
Lane 2: NIH:OVCAR-3 (Human ovarian adenocarcinoma cell) cell lysate
Lane 3: LNCaP (Human prostate cancer cell) cell lysate
Lane 4: HUVEC (Human umbilical vein endothelial cell) cell lysate
Lane 5: MOLT-4 (Human acute T-Cell leukemia cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
MOLT-4 is a negative control (PMID: 30231940).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724777, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 134 kDa
Observed band size: 150-180 kDa -
Western blot analysis of Jagged1 on different lysates with Rabbit anti-Jagged1 antibody (HA724777) at 1/5,000 dilution.
Lane 1: Hepa1-6 (Mouse liver cancer cell) cell lysate
Lane 2: bEnd.3 (Mouse brain microvascular endothelial cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724777, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 134 kDa
Observed band size: 150-180 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"