KCNK4 / K2P4.1 Rabbit Polyclonal Antibody
Catalog# ER1803-07
KCNK4 / K2P4.1 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Mouse
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Rat
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unconjugated
Overview
Product Name
KCNK4 / K2P4.1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human K2P41 aa 326-375 / 393.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
Mouse colon tissue lysate, mouse brain tissue lysate, rat colon tissue lysate, rat brain tissue lysate, rat large intestine tissue, mouse colon tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:50-1:200
Target
Function
K2P4.1 (also named TWIK-related arachidonic acid stimulated K+ channel, TRAAK or KCNK4) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 15 members. These channels show little time or voltage dependence and are considered to be “leaky” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini.
Background References
1. Brohawn S G et al. Crystal structure of the human K2P TRAAK, a lipid- and mechano-sensitive K+ ion channel. Science 335:436-441 (2012).
2. Brohawn S G et al. Physical mechanism for gating and mechanosensitivity of the human TRAAK K+ channel. Nature 516:126-130 (2014).
Sequence Similarity
Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
Post-translational Modification
N-glycosylated.
Subcellular Location
Cell membrane.
Synonyms
K2p4.1 antibody
K2P4.1 potassium channel antibody
KCNK 4 antibody
MGC144821 antibody
Potassium channel subfamily K member 4 antibody
Potassium inwardly rectifying channel subfamily K member 4 antibody
TRAAK antibody
TRAAK1 antibody
TRAAKt antibody
TWIK related arachidonic acid stimulated potassium channel protein antibody
ExpandImages
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Western blot analysis of KCNK4 / K2P4.1 on different lysates with Rabbit anti-KCNK4 / K2P4.1 antibody (ER1803-07) at 1/2,000 dilution.
Lane 1: Mouse colon tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat colon tissue lysate
Lane 4: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 50 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-07) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-K2P4.1 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"