KBTBD8 Mouse Monoclonal Antibody [6G-8A]
Catalog# EM1706-50
KBTBD8 Mouse Monoclonal Antibody [6G-8A]
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WB
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FC
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Human
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unconjugated
Overview
Product Name
KBTBD8 Mouse Monoclonal Antibody [6G-8A]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
69 kDa
Positive Control
Human KBTBD8 recombinant protein, KBTBD8-hIgGFc transfected HEK293 cell lysate, A431, Jurkat, Hela, K562, Hek293.
Conjugation
unconjugated
Clone Number
6G-8A
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:50-1:100
Target
Function
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of neural crest specification (PubMed:26399832). The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1: monoubiquitination promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.
Background References
1. Lührig S et al. The novel BTB-kelch protein, KBTBD8, is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis. Cell Div 11 8(1):3 (2013).
2. Kim JJ et al. Identification of 15 loci influencing height in a Korean population. J Hum Genet 55(1):27-31 (2010).
Subcellular Location
Cytoplasm, cytoskeleton
UNIPROT
Synonyms
AU040152 antibody
FLJ57592 antibody
KBTB8_HUMAN antibody
Kbtbd8 antibody
Kelch repeat and BTB (POZ) domain containing 8 antibody
Kelch repeat and BTB domain containing protein 8 antibody
Kelch repeat and BTB domain-containing protein 8 antibody
KIAA1842 antibody
MGC91215 antibody
mKIAA1842 antibody
ExpandImages
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Western blot analysis of KBTBD8 on HEK293 (1) and KBTBD8-hIgGFc transfected HEK293 (2) cell lysate using anti-KBTBD8 antibody at 1/1,000 dilution.
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Western blot analysis of KBTBD8 on different cell lysate using anti-KBTBD8 antibody at 1/1,000 dilution.
Positive control: Line1: A431 Line2: Jurkat Line3: Hela Line4: K562 Line5: Hek293
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"