KIF3A Rabbit Polyclonal Antibody
Catalog# ER1803-40
KIF3A Rabbit Polyclonal Antibody
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
KIF3A Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human KIF3A aa 154-364 / 699.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 80 kDa
Positive Control
Rat testis tissue, Daudi, rat cerebellum tissue, mouse testis tissue, A549.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:100
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FC
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1:50-1:100
Target
Function
Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitement of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.
Background References
1. Kodani A et al. Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages. EMBO J 32:597-607 (2013).
2. Kinzel D et al. Pitchfork regulates primary cilia disassembly and left-right asymmetry. Dev Cell 19:66-77 (2010).
Sequence Similarity
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily.
Subcellular Location
Cytoskeleton.
Synonyms
KIF 3 antibody
KIF 3A antibody
KIF3 antibody
Kif3a antibody
KIF3A_HUMAN antibody
Kifl antibody
Kinesin family member 3A antibody
Kinesin family protein 3 antibody
Kinesin family protein 3A antibody
Kinesin like protein KIF 3A antibody
ExpandImages
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Western blot analysis of KIF3A on Mouse testis tissue lysates with Rabbit anti-KIF3A antibody (ER1803-40) at 1/2,000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1803-40, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 80 kDa
Observed band size: 80 kDa -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-KIF3A antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-KIF3A antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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