LAT Recombinant Rabbit Monoclonal Antibody [PSH09-76] - BSA and Azide free
Catalog# HA751318
LAT Recombinant Rabbit Monoclonal Antibody [PSH09-76] - BSA and Azide free
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WB
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IF-Cell
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FC
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Human
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HA723141
含抗保成分
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unconjugated
Overview
Product Name
LAT Recombinant Rabbit Monoclonal Antibody [PSH09-76] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human LAT aa 28-262.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 28 kDa
Positive Control
Jurkat cell lysate, MoLT-4 cell lysate, Jurkat.
Conjugation
unconjugated
Clone Number
PSH09-76
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:10,000
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FC
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1:20,000
Target
Function
The Linker for activation of T cells, also known as linker of activated T cells or LAT, is a protein involved in the T-cell antigen receptor signal transduction pathway which in humans is encoded by the LAT gene. Alternative splicing results in multiple transcript variants encoding different isoforms. The LAT protein encoded by the gene of the same name, plays a key role in the diversification of T cell signaling pathways following activation of the T-cell antigen receptor (TCR) signal transduction pathway, which is first catalyzed by TCR binding to MHC class II. LAT is a transmembrane protein localizes to lipid rafts (also known as glycosphingolipid-enriched microdomains or GEMs) and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. In mouse thymocytes, lack of functional LAT or the inability for LAT to be phosphorylated leads to complete lack of T cell development. Moreover, mutation and deletion of LAT hampers overall TCR mediated T cell response.
Background References
1. Arkee T et al. TRAF3 in T Cells Restrains Negative Regulators of LAT to Promote TCR/CD28 Signaling. J Immunol. 2021 Jul
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
36 kDa phospho tyrosine adapter protein antibody
36 kDa phospho-tyrosine adapter protein antibody
36 kDa phospho-tyrosine adaptor protein antibody
LAT 1 antibody
lat antibody
LAT_HUMAN antibody
LAT1 antibody
Linker for activation of T cells antibody
Linker for activation of T cells family member 1 antibody
linker for activation of T cells, transmembrane adaptor antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of LAT on different lysates with Rabbit anti-LAT antibody (HA751318) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Daudi cell lysate (negative)
Lane 3: MoLT-4 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 36 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751318) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of Jurkat (positive) and Raji (negative) labeling LAT with Rabbit anti-LAT antibody (HA751318) at 1/10,000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LAT antibody (HA751318) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Relative expression (RE)
Flow cytometric analysis of Raji (left, negative) and Jurkat (right, positive) cells labeling LAT.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA751318, 1/20,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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