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LDHA Mouse Monoclonal Antibody [20-179]

Usd: 350

Specification

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Catalog# M1007-7

LDHA Mouse Monoclonal Antibody [20-179]

Application

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

LDHA Mouse Monoclonal Antibody [20-179]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human LDHA aa 1-50 / 332.

Species Reactivity

Human, Mouse

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 37 kDa

Positive Control

Mouse testis tissue, human kidney tissue.

Conjugation

unconjugated

Clone Number

20-179

RRID

AB_3073022

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:50-1:100

Target

Function

Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.

Background References

1. Singer P.A., Trevor K., Oshima R.G. J. Biol. Chem. 261:538-547(1986).

2. Ichinose Y., Morita T., Zhang F. etc. Gene 70:85-95(1988).

3. Dixon M.J., Robinson V., White A., Ferguson M.W. J. Anat. 183:423-438(1993).

Sequence Similarity

Belongs to the LDH/MDH superfamily. LDH family.

Post-translational Modification

ISGylated.

Subcellular Location

Cytoplasm

UNIPROT

SwissProt: P00338 Human

SwissProt: P06151 Mouse

Synonyms

Cell proliferation-inducing gene 19 protein antibody

GSD11 antibody

L lactate dehydrogenase A chain antibody

L-lactate dehydrogenase A chain antibody

l7R2 antibody

Lactate dehydrogenase 1, A chain antibody

Lactate dehydrogenase A antibody

Lactate dehydrogenase A4 antibody

Lactate dehydrogenase M antibody

LDH A antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-LDHA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1007-7" style="font-weight: bold;text-decoration: underline;">M1007-7</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-LDHA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LDHA antibody (<a href="/products/M1007-7" style="font-weight: bold;text-decoration: underline;">M1007-7</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1007-7" style="font-weight: bold;text-decoration: underline;">M1007-7</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LDHA antibody (M1007-7) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1007-7) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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