LDL Receptor Rabbit Polyclonal Antibody
Catalog# R1512-12
LDL Receptor Rabbit Polyclonal Antibody
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WB
-
IF-Cell
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FC
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Human
-
Mouse
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unconjugated
Overview
Product Name
LDL Receptor Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human LDL Receptor aa 377-441.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 95 kDa
Positive Control
Hela cell lysates, MCF-7 cell lysates, A549, PC-3M, human liver cancer tissue, mouse testis tissue, Hela.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-2000
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IF-cell
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1:100
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FC
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1μg/Test
Target
Function
LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family, which includes LDLR, LRP, Megalin, VLDLR, and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats, and an O-linked sugar domain. The LDL receptor is a cell surface transmembrane protein that mediates the uptake of low density lipoprotein and its degradation in the lysosome, which provides cholesterol to cells. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. Mutations in LDLR cause the autosomal dominant disease, familial hypercholesterolemia (FH), which promotes premature coronary atherosclerosis.
Background References
暂无
Sequence Similarity
Belongs to the LDLR family.
Post-translational Modification
N- and O-glycosylated.; Ubiquitinated by MYLIP leading to degradation.
Subcellular Location
Golgi apparatus, Plasma membrane, Endosome, Lysosome, Cell membrane.
Synonyms
FH antibody
FHC antibody
LDL R antibody
LDL receptor antibody
LDLCQ2 antibody
Ldlr antibody
LDLR_HUMAN antibody
Low Density Lipoprotein Receptor antibody
Low density lipoprotein receptor class A domain containing protein 3 antibody
Low density lipoprotein receptor familial hypercholesterolemia antibody
ExpandImages
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Sample:Human colon Lysate at 45ug;
Primary: Anti-LDL receptor (R1512-12) at 1:300 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 5000 dilution;
Predicted band size : 92kD
Observed band size : 92kD -
Sample:
K562(Human) Cell Lysate at 30 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
SW480(Human) Cell Lysate at 30 ug
Primary: Anti-LDL receptor (R1512-12) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kD
Observed band size: 120 kD -
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min; Antibody incubation with (LDL receptor) polyclonal Antibody, Unconjugated (R1512-12) 1:100, 90 minutes at 37℃; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37℃ for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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