LGI1 Recombinant Rabbit Monoclonal Antibody [JE54-88]
Catalog# ET7110-86
LGI1 Recombinant Rabbit Monoclonal Antibody [JE54-88]
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
LGI1 Recombinant Rabbit Monoclonal Antibody [JE54-88]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human LGI1 aa 130-270 / 557.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
64 kDa
Positive Control
A431 cell lysate, A172 cell lysate, MG-63 cell lysate, mouse hippocampus tissue lysate.
Conjugation
unconjugated
Clone Number
JE54-88
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
Target
Function
Leucine-rich, glioma inactivated 1, also known as LGI1, is a protein which in humans is encoded by the LGI1 gene. It may be a metastasis suppressor. The leucine-rich glioma inactivated -1 gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobic segment representing a putative transmembrane domain with the amino terminus located outside the cell. It also contains leucine-rich repeats with conserved cysteine-rich flanking sequences. This gene is predominantly expressed in neural tissues and its expression is reduced in low grade brain tumors and significantly reduced or absent in malignant gliomas. Since its earliest discovery, the LGI1 gene has been implicated in the control of cancer metastasis and in a predisposition to epilepsy. Following genetic linkage studies placing the hereditary form of autosomal dominant partial epilepsy with auditory features (ADPEAF) on chromosome region 10q24 mutation analysis of affected members in these families demonstrated LGI1 was a major cause of the disease.
Background References
1. Binks. et. al. LGI1, CASPR2 and related antibodies: a molecular evolution of the phenotypes. J Neurol Neurosurg Psychiatry. 2018 May.
2. van Sonderen A. et. al. The value of LGI1, Caspr2 and voltage-gated potassium channel antibodies in encephalitis. Nat Rev Neurol. 2017 May.
Tissue Specificity
Predominantly expressed in neural tissues, especially in brain. Expression is reduced in low-grade brain tumors and significantly reduced or absent in malignant gliomas. Isoform 1 is absent in the cerebellum and is detectable in the occipital cortex and hippocampus; higher amounts are observed in the parietal and frontal cortices, putamen, and, particularly, in the temporal neocortex, where it is 3.5 times more abundant than in the hippocampus (at protein level). Isoform 3 shows the highest expression in the occipital cortex and the lowest in the hippocampus (at protein level).
Post-translational Modification
Glycosylated.
Subcellular Location
Secreted, synapse.
Synonyms
ADLTE antibody
ADPAEF antibody
ADPEAF antibody
Epitempin 1 antibody
EPITEMPIN antibody
Epitempin-1 antibody
EPT antibody
ETL1 antibody
IB1099 antibody
leucine rich glioma inactivated 1 antibody
ExpandImages
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Western blot analysis of LGI1 on different lysates with Rabbit anti-LGI1 antibody (ET7110-86) at 1/500 dilution.
Lane 1: A431 cell lysate
Lane 2: A172 cell lysate
Lane 3: MG-63 cell lysate
Lane 4: Mouse hippocampus tissue lysate(20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-86) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"