LIFR Recombinant Rabbit Monoclonal Antibody [PSH08-76] - BSA and Azide free
Catalog# HA751241
LIFR Recombinant Rabbit Monoclonal Antibody [PSH08-76] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
LIFR Recombinant Rabbit Monoclonal Antibody [PSH08-76] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human LIFR aa 1-850.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 124 kDa
Positive Control
HeLa cell lysate, PANC-1 cell lysate, MDA-MB-231 cell lysate, HCT 116 cell lysate, RD cell lysate.
Conjugation
unconjugated
Clone Number
PSH08-76
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
LIFR also known as CD118 (Cluster of Differentiation 118), is a subunit of a receptor for leukemia inhibitory factor. The leukemia inhibitory factor (LIF) is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIF action appears to be mediated through a high-affinity receptor complex composed of a low-affinity LIF binding chain (LIF receptor) and a high-affinity converter subunit, glycoprotein 130 (IL6ST, gp130). Both LIFR and gp130 are members of a family of cytokine receptors that includes components of the receptors for the majority of hematopoietic cytokines and for cytokines that affect other systems, including the ciliary neurotrophic factor, growth hormone and prolactin.
Background References
1. Viswanadhapalli S et al. Targeting LIF/LIFR signaling in cancer. Genes Dis. 2021 Apr
2. Bai Z et al. Long Noncoding RNA LIFR-AS1: A New Player in Human Cancers. Biomed Res Int. 2022 Jan
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD118 antibody
CD118 antigen antibody
FLJ98106 antibody
FLJ99923 antibody
Leukemia inhibitory factor receptor alpha antibody
Leukemia inhibitory factor receptor antibody
LIF R antibody
LIF receptor antibody
LIF-R antibody
Lifr antibody
ExpandImages
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☑ Relative expression (RE)
This data was developed using HA723028, the same antibody clone in a different buffer formulation.
Western blot analysis of LIFR on different lysates with Rabbit anti-LIFR antibody (HA723028) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: T-47D cell lysate (negative)
Lane 4: MDA-MB-231 cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: RD cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 124 kDa
Observed band size: 180 kDa
Exposure time: 46 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723028) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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