LILRB1 Recombinant Rabbit Monoclonal Antibody [PSH04-59]
Catalog# HA722158
LILRB1 Recombinant Rabbit Monoclonal Antibody [PSH04-59]
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WB
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Human
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unconjugated
Overview
Product Name
LILRB1 Recombinant Rabbit Monoclonal Antibody [PSH04-59]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human LILRB1 aa 1-461 / 650.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 71 kDa
Positive Control
Raji cell lysate, Daudi cell lysate, THP-1 cell lysate.
Conjugation
unconjugated
Clone Number
PSH04-59
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Leukocyte immunoglobulin-like receptor subfamily B member 1 is a protein that in humans is encoded by the LILRB1 gene. This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
Background References
1. Xu X et al. LILRB1+ immune cell infiltration identifies immunosuppressive microenvironment and dismal outcomes of patients with ovarian cancer. Int Immunopharmacol. 2023 Jun
2. Zhang Y et al. Poor Prognosis and Therapeutic Responses in LILRB1-Expressing M2 Macrophages-Enriched Gastric Cancer Patients. Front Oncol. 2021 Aug
Subcellular Location
Cell membrane; Secreted.
UNIPROT
Synonyms
CD85 antibody
CD85 antigen antibody
CD85 antigen like family member J antibody
CD85 antigen-like family member J antibody
CD85j antibody
CD85j antigen antibody
Ig like transcript 2 antibody
ILT 2 antibody
ILT-2 antibody
ILT2 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of LILRB1 on different lysates with Rabbit anti-LILRB1 antibody (HA722158) at 1/1,000 dilution.
Lane 1: Raji cell lysate
Lane 2: Daudi cell lysate
Lane 3: THP-1 cell lysate
Lane 4: A431 cell lysate (negative)
Lane 5: K-562 cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 120 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722158) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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