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LKB1 Recombinant Rabbit Monoclonal Antibody [PSH09-29] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751283

LKB1 Recombinant Rabbit Monoclonal Antibody [PSH09-29] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

LKB1 Recombinant Rabbit Monoclonal Antibody [PSH09-29] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human LKB1 aa 1-400.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 49 kDa

Positive Control

K-562 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

PSH09-29

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:1,000

  • IP

  • 1-2μg/sample

Target

Function

The STK11/LKB1 gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumour suppressor. LKB1 is a primary upstream kinase of adenosine monophosphate-activated protein kinase (AMPK), a necessary element in cell metabolism that is required for maintaining energy homeostasis. It is now clear that LKB1 exerts its growth suppressing effects by activating a group of ~14 other kinases, comprising AMPK and AMPK-related kinases. Activation of AMPK by LKB1 suppresses growth and proliferation when energy and nutrient levels are scarce. Activation of AMPK-related kinases by LKB1 plays vital roles maintaining cell polarity thereby inhibiting inappropriate expansion of tumour cells. A picture from current research is emerging that loss of LKB1 leads to disorganization of cell polarity and facilitates tumour growth under energetically unfavorable conditions. A study in rats showed that LKB1 expression is upregulated in cardiomyocytes after birth and that LKB1 abundance negatively correlates with proliferation of neonatal rat cardiomyocytes.

Background References

1. Compton SE et al. LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation. Mol Cell. 2023 Jun

2. Pons-Tostivint E et al. STK11/LKB1 Modulation of the Immune Response in Lung Cancer: From Biology to Therapeutic Impact. Cells. 2021 Nov

Subcellular Location

Nucleus, Cytoplasm, Membrane, Mitochondrion.

UNIPROT

SwissProt: Q15831 Human

SwissProt: Q9WTK7 Mouse

SwissProt: D4AE59 Rat

Synonyms

hLKB1 antibody

Liver kinase B1 antibody

LKB1 antibody

PJS antibody

Polarization related protein LKB1 antibody

Renal carcinoma antigen NY-REN-19 antibody

Serine/Threonine Kinase 11 antibody

Serine/threonine protein kinase 11 antibody

Serine/threonine protein kinase LKB1 antibody

Serine/threonine protein kinase STK11 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of LKB1 on different lysates with Rabbit anti-LKB1 antibody (<a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a>) at 1/2,000 dilution.<br /><br />Lane 1: K-562 cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: HeLa cell lysate (negative) (20 µg/Lane)<br />Lane 4: HEK-293 cell lysate (20 µg/Lane)<br />Lane 5: COS-1 cell lysate (20 µg/Lane)<br />Lane 6: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 7: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 8: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 49 kDa<br />Observed band size: 56/50 kDa<br /><br />Exposure time: 20 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of LKB1 on different lysates with Rabbit anti-LKB1 antibody (HA751283) at 1/2,000 dilution.

    Lane 1: K-562 cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: HeLa cell lysate (negative) (20 µg/Lane)
    Lane 4: HEK-293 cell lysate (20 µg/Lane)
    Lane 5: COS-1 cell lysate (20 µg/Lane)
    Lane 6: Mouse brain tissue lysate (40 µg/Lane)
    Lane 7: Mouse testis tissue lysate (40 µg/Lane)
    Lane 8: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 49 kDa
    Observed band size: 56/50 kDa

    Exposure time: 20 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751283) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LKB1 antibody (<a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LKB1 antibody (HA751283) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751283) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-LKB1 antibody (<a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-LKB1 antibody (HA751283) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751283) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-LKB1 antibody (<a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-LKB1 antibody (HA751283) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751283) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • LKB1 was immunoprecipitated from 0.2 mg HEK-293 cell lysate with <a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a> at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HEK-293 cell lysate (input)<br />Lane 2: <a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a> IP in HEK-293 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA751283" style="font-weight: bold;text-decoration: underline;">HA751283</a> in HEK-293 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    LKB1 was immunoprecipitated from 0.2 mg HEK-293 cell lysate with HA751283 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751283 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HEK-293 cell lysate (input)
    Lane 2: HA751283 IP in HEK-293 cell lysate
    Lane 3: Rabbit IgG instead of HA751283 in HEK-293 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 3 minutes; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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LKB1 Recombinant Rabbit Monoclonal Antibody [PSH09-29]

Application: WB,IHC-P,IP

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated