LMO2 Recombinant Rabbit Monoclonal Antibody [PD00-25]
Catalog# HA721212
LMO2 Recombinant Rabbit Monoclonal Antibody [PD00-25]
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WB
-
IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
LMO2 Recombinant Rabbit Monoclonal Antibody [PD00-25]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein full length human LMO2.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 18 kDa
Positive Control
K562 cell lysates, mouse brain tissue, human tonsil tissue, human lymph nodes tissue.
Conjugation
unconjugated
Clone Number
PD00-25
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000-1:2000
Target
Function
The LIM-domain-only (LMO) family of proteins function as linkers in the co-regulation of nuclear transcription by mediating protein-protein interactions. The four members of the LMO family collectively have significant roles in cell fate determination, cell growth and differentiation, and organ development. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms. LMO2 has a particular function in normal and lymphatic endothelial cells involving the regulation of angiogenesis and lymphangiogenesis. Immunohistochemical studies have also demonstrated expression of LMO2 in both normal germinal center B-cells and germinal center-derived B-cell lymphomas, including follicular lymphoma and diffuse large B-cell lymphoma. The use of anti-LMO2 is valuable as a tool in the identification of lymphomas of B-cell origin.
Background References
1. Hirano KI. et. al. LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice. Elife. 2021 Aug
2. Wang W. et. al. Biochemical Feature of LMO2 Interactome and LMO2 Function Prospect. Med Sci Monit Basic Res. 2020 Jul
Subcellular Location
Nucleus.
Synonyms
Cysteine rich protein TTG 2 antibody
Cysteine rich protein TTG2 antibody
Cysteine-rich protein TTG-2 antibody
LIM domain only 2 (rhombotin like 1) antibody
LIM domain only 2 antibody
LIM domain only protein 2 antibody
LMO 2 antibody
LMO-2 antibody
lmo2 antibody
RBTN 2 antibody
ExpandImages
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Western blot analysis of LMO2 on K562 cell lysates with Rabbit anti-LMO2 antibody (HA721212) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721212) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LMO2 antibody (HA721212) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721212) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LMO2 antibody (HA721212) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721212) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-LMO2 antibody (HA721212) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721212) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"