LNP Mouse Monoclonal Antibody [7-3]
Catalog# EM1701-47
LNP Mouse Monoclonal Antibody [7-3]
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
LNP Mouse Monoclonal Antibody [7-3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within mouse LNP aa 198-425 / 425.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC-P, IF-Cell
Target Molecular Weight
Predicted band size: 48 kDa
Positive Control
SHG-44, rat brain tissue, rat spinal cord tissue, mouse brain tissue.
Conjugation
unconjugated
Clone Number
7-3
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
Endoplasmic reticulum (ER)-shaping membrane protein that plays a role in determining ER morphology. Involved in the stabilization of nascent three-way ER tubular junctions within the ER network. May also play a role as a curvature-stabilizing protein within three-way ER tubular junction network. May be involved in limb and central nervous system development
Background References
1. Spitz F et al. A global control region defines a chromosomal regulatory landscape containing the HoxD cluster. Cell 113:405-417 (2003).
2. Kmita, M et al. Evolutionary conserved sequences are required for the insulation of the vertebrate HoxD complex in neural cells. Development. 129: 5521–5528 (2002).
Sequence Similarity
Belongs to the lunapark family.
Tissue Specificity
Expressed in neural precursor cells, where it is detected at the growth-cone-like structure and branching sites of neurite-like processes.
Post-translational Modification
Myristoylated; myristoylation is necessary for the endoplasmic reticulum (ER) three-way ER tubular junction formation, but is not required neither for membrane translocation, membrane topology formation, nor for the specific localization to ER membranes.; Phosphorylated. Phosphorylation occurs at Ser-177, Ser-182, Ser-217, Ser-227, Ser-321 and Ser-384 during interphase. Phosphorylation occurs at Ser-114, Ser-153, Ser-194, Thr-211 and Ser-353 during mitosis; these phosphorylations reduce both its homodimerization and the ER three-way tubular junction formation.; Subject to proteasomal degradation following phosphorylation during mitosis.
Subcellular Location
Endoplasmic reticulum.
Synonyms
lnp antibody
LNP_HUMAN antibody
Protein lunapark antibody
Images
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ICC staining LNP (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-LNP antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-LNP antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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