LOX-1 Mouse Monoclonal Antibody [2G5]
Catalog# RT1367
LOX-1 Mouse Monoclonal Antibody [2G5]
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WB
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IP
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IF
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
LOX-1 Mouse Monoclonal Antibody [2G5]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
peptide
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP, IF, IHC-P
Molecular Weight
40 kDa
Positive Control
WI-38, MCF7, HepG2, HepG2.
Conjugation
unconjugated
Clone Number
2G5
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃
Storage Buffer
1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:100-1:1,000
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IP
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1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
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IF
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1:50-1:500
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IHC-P
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1:50-1:500
Target
Function
The lysyl oxidase family of extracellular proteins includes LOX and four LOX-like enzymes, which are responsible for the deamination of peptidyl lysine residues of collagens and elastin. They also catalyze inter- and intra-crosslinking reactions. Overexpression of LOX may cause severe fibrotic degeneration due to its high resistance to degradative enzymes. Procollagen C-proteinase activity processes LOX from a precursor protein to a mature form. Activation of LOX occurs in normal developing and adult skin, and alterations in LOX expression and activity are associated with skin aging and senescence. LOX is crucial for development of the cardiovascular and respiratory systems. In addition, LOX plays a role in cancer, wound healing, as well as cell motility, chemotaxis and differentiation.
Background References
1. Chen, L.J., et al. 2005. Downregulation of lysyl oxidase and upregulation of cellular thiols in rat fetal lung fibroblasts treated with cigarette smoke condensate. Toxicol. Sci. 83: 372-379.
2. Goto, Y., et al. 2005. Transforming growth factor β-1-mediated upregulation of lysyl oxidase in the kidneys of hereditary nephrotic mouse with chronic renal fibrosis. Virchows Arch. 447: 859-868.
Subcellular Location
Secreted
UNIPROT
Synonyms
lox antibody
LYOX antibody
LYOX_HUMAN antibody
Lysyl oxidase antibody
MGC105112 antibody
Protein lysine 6 oxidase antibody
Protein-lysine 6-oxidase antibody
Images
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Western blot analysis of LOX expression in WI-38 (A) and MCF7 (B) whole cell lysates.
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Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic and nuclear staining of epidermal cells (A). Immunofluorescence staining of formalinfixed HepG2 cells showing cell surface localization (B).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"