Langerin / CD207 Recombinant Rabbit Monoclonal Antibody [JE32-54]
Catalog# HA722427
Langerin / CD207 Recombinant Rabbit Monoclonal Antibody [JE32-54]
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
Langerin / CD207 Recombinant Rabbit Monoclonal Antibody [JE32-54]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant fragment within Human Langerin aa 200 to the C-terminus.
Species Reactivity
Human, Mouse
Validated Applications
IHC-P
Molecular Weight
Predicted band size: 37 kDa
Positive Control
Human esophagus tissue, human skin tissue, mouse skin tissue.
Conjugation
unconjugated
Clone Number
JE32-54
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-P
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1:200-1:1,000
Target
Function
Langerin (CD207) is a type II transmembrane protein which is encoded by the CD207 gene in humans. It was discovered by scientists Sem Saeland and Jenny Valladeau as a main part of Birbeck granules. Langerin is C-type lectin receptor on Langerhans cells (LCs) and in mice also on dermal interstitial CD103+ dendritic cells (DC) and on resident CD8+ DC in lymph nodes.
Background References
1. Zhang D et al. CD207 Expression Level is a New Prognostic Marker for Condyloma Acuminatum. Clin Cosmet Investig Dermatol. 2023 Jun
2. Olexen CM et al. Monitoring Circulating CD207(+)CD1a(+) Cells in Langerhans Cell Histiocytosis and Clinical Implications. J Immunol. 2022 Jul
Subcellular Location
Membrane.
Synonyms
C type lectin domain family 4 member K antibody
C-type lectin domain family 4 member K antibody
CD 207 antibody
CD207 antibody
CD207 antigen antibody
CD207 antigen, langerin antibody
CD207 molecule antibody
CLC4K_HUMAN antibody
CLEC 4K antibody
CLEC4K antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-Langerin / CD207 antibody (HA722427) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722427) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Langerin / CD207 antibody (HA722427) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722427) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Langerin / CD207 antibody (HA722427) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722427) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human brain tissue (negative) with Rabbit anti-Langerin / CD207 antibody (HA722427) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722427) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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