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Livin Recombinant Rabbit Monoclonal Antibody [PSH12-72] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751448

Livin Recombinant Rabbit Monoclonal Antibody [PSH12-72] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Livin Recombinant Rabbit Monoclonal Antibody [PSH12-72] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Livin aa 1-250.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP

Target Molecular Weight

Predicted band size: 33 kDa

Positive Control

SK-MEL-28 cell lysate, human breast cancer tissue, human placenta tissue.

Conjugation

unconjugated

Clone Number

PSH12-72

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

BIRC7, also known as Livin, is a member of the inhibitor of apoptosis protein (IAP) family. It plays a dual role as an apoptotic regulator, capable of exerting both proapoptotic and anti-apoptotic activities. Livin is crucial for controlling apoptosis, cell proliferation, and cell cycle regulation. Its anti-apoptotic function is primarily mediated through the inhibition of caspases, including CASP3, CASP7, and CASP9, as well as through its E3 ubiquitin-protein ligase activity. Livin promotes cell survival by ubiquitinating and targeting DIABLO/SMAC for degradation, thereby preventing DIABLO/SMAC from disrupting XIAP/BIRC4-caspase interactions. Livin protects cells from apoptosis induced by TNF or chemical agents such as adriamycin, etoposide, and staurosporine. This anti-apoptotic effect is achieved through the activation of MAPK8/JNK1 and possibly MAPK9/JNK2, which depends on TAB1 and MAP3K7/TAK1. In vitro, Livin inhibits CASP3 and the proteolytic activation of pro-CASP9. Structurally, Livin contains a single baculoviral IAP repeat (BIR) domain and a RING domain at the C-terminus. Overexpression of Livin has been observed in various cancers, including lung, colon, and prostate cancers, making it a potential therapeutic target.

Background References

1. Kasof GM, Gomes BC. Livin, a novel inhibitor of apoptosis protein family member. J Biol Chem. 2001 Feb.

2. Sanna MG, et al. IAP suppression of apoptosis involves distinct mechanisms: the TAK1/JNK1 signaling cascade and caspase inhibition. Mol Cell Biol. 2002 Mar.

Subcellular Location

Nucleus, Cytoplasm, Golgi apparatus

UNIPROT

SwissProt: Q96CA5 Human

Synonyms

Baculoviral IAP repeat containing 7 antibody

Baculoviral IAP repeat containing protein 7 antibody

Baculoviral IAP repeat-containing protein 7 antibody

BIRC 7 antibody

birc7 antibody

BIRC7_HUMAN antibody

KIAP antibody

Kidney inhibitor of apoptosis protein antibody

Livin antibody

Livin inhibitor of apotosis antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />This data was developed using <a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of Livin on different lysates with Rabbit anti-Livin antibody (<a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a>) at 1/5,000 dilution.<br /><br />Lane 1: SK-MEL-28 cell lysate<br />Lane 2: HeLa cell lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    This data was developed using HA723483, the same antibody clone in a different buffer formulation.
    Western blot analysis of Livin on different lysates with Rabbit anti-Livin antibody (HA723483) at 1/5,000 dilution.

    Lane 1: SK-MEL-28 cell lysate
    Lane 2: HeLa cell lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 35 kDa

    Exposure time: 30 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723483) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • This data was developed using <a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Livin antibody (<a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA723483, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Livin antibody (HA723483) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723483) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Livin antibody (<a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA723483, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Livin antibody (HA723483) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723483) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a>, the same antibody clone in a different buffer formulation.<br />Livin was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with <a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a> at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: SK-MEL-28 cell lysate (input)<br />Lane 2: <a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a> IP in SK-MEL-28 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723483" style="font-weight: bold;text-decoration: underline;">HA723483</a> in SK-MEL-28 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 12 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    This data was developed using HA723483, the same antibody clone in a different buffer formulation.
    Livin was immunoprecipitated from 0.2 mg SK-MEL-28 cell lysate with HA723483 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723483 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: SK-MEL-28 cell lysate (input)
    Lane 2: HA723483 IP in SK-MEL-28 cell lysate
    Lane 3: Rabbit IgG instead of HA723483 in SK-MEL-28 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 12 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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