LNP Mouse Monoclonal Antibody [11-1]
Catalog# EM1701-57
LNP Mouse Monoclonal Antibody [11-1]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
LNP Mouse Monoclonal Antibody [11-1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within mouse LNP aa 198-425 / 425.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 48 kDa
Positive Control
K-562 cell lysate, SH-SY5Y cell lysate, SiHa cell lysate, mouse brain tissue lysate, mouse testis tissue lysate, rat brain tissue lysate, rat testis tissue lysate, human brain tissue, human testis tissue, mouse brain tissue, mouse testis tissue, rat brain tissue, rat testis tissue, rat spinal cord tissue.
Conjugation
unconjugated
Clone Number
11-1
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:500-1:2,000
Target
Function
Endoplasmic reticulum (ER)-shaping membrane protein that plays a role in determining ER morphology. Involved in the stabilization of nascent three-way ER tubular junctions within the ER network. May also play a role as a curvature-stabilizing protein within the three-way ER tubular junction network. May be involved in limb development (By similarity). Is involved in central nervous system development. Enables identical protein binding activity. Involved in endoplasmic reticulum tubular network maintenance and positive regulation of endoplasmic reticulum tubular network organization. Located in endoplasmic reticulum tubular network membrane and nucleoplasm. Is integral component of endoplasmic reticulum membrane.
Background References
1. Moriya K et al. Protein N-myristoylation plays a critical role in the endoplasmic reticulum morphological change induced by overexpression of protein Lunapark, an integral membrane protein of the endoplasmic reticulum. PLoS ONE 8:E78235-E78235 (2013).
2. Shemesh T et al. A model for the generation and interconversion of ER morphologies. Proc Natl Acad Sci U.S.A. 111:E5243-E5251 (2014).
Sequence Similarity
Belongs to the lunapark family.
Tissue Specificity
Expressed in most tissues at basal level, with reinforcement in distal limb buds, genital bud, and in parts of the central nervous system.
Post-translational Modification
Myristoylated; myristoylation is necessary for the endoplasmic reticulum (ER) three-way ER tubular junction formation, but is not required neither for membrane translocation, membrane topology formation, nor for the specific localization to ER membranes.; Phosphorylated. Phosphorylation occurs at Ser-177, Ser-182, Ser-222, Ser-316 and Ser-380 during interphase. Phosphorylation occurs at Ser-114, Ser-153, Ser-194, Thr-211 and Ser-348 during mitosis; these phosphorylations reduce both its homodimerization and the ER three-way tubular junction formation.; Subject to proteasomal degradation following phosphorylation during mitosis.
Subcellular Location
Endoplasmic reticulum membrane.
Synonyms
lnp antibody
LNP_HUMAN antibody
Protein lunapark antibody
Endoplasmic reticulum junction formation protein lunapark
ER junction formation factor lunapark
LNPK
KIAA1715
LNP
Images
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Western blot analysis of LNP on different lysates with Mouse anti-LNP antibody (EM1701-57) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: SiHa cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse testis tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 55 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-57) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-LNP antibody (EM1701-57) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-57) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-LNP antibody (EM1701-57) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-LNP antibody (EM1701-57) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-LNP antibody (EM1701-57) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-LNP antibody (EM1701-57) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Mouse anti-LNP antibody (EM1701-57) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Mouse anti-LNP antibody (EM1701-57) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-57) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"