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Lyn Recombinant Rabbit Monoclonal Antibody [JE44-75]

Usd: 385

Specification

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Catalog# HA721991

Lyn Recombinant Rabbit Monoclonal Antibody [JE44-75]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Cynomolgus monkey

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Lyn Recombinant Rabbit Monoclonal Antibody [JE44-75]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Lyn aa 1-100.

Species Reactivity

Human, Mouse, Rat, Cynomolgus monkey

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 59 kDa

Positive Control

Ramos cell lysate, A431 cell lysate, Jurkat cell lysate, THP-1 cell lysate, Raji cell lysate, K-562 cell lysate, U-937 cell lysate, mouse lung tissue lysate, rat lung tissue lysate, A431.

Conjugation

unconjugated

Clone Number

JE44-75

RRID

AB_3096800

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:1,000

Target

Function

Tyrosine-protein kinase Lyn is a protein that in humans is encoded by the LYN gene. Lyn is a member of the Src family of protein tyrosine kinases, which is mainly expressed in hematopoietic cells, in neural tissues liver, and adipose tissue. In various hematopoietic cells, Lyn has emerged as a key enzyme involved in the regulation of cell activation. In these cells, a small amount of LYN is associated with cell surface receptor proteins, including the B cell antigen receptor (BCR), CD40, or CD19. The abbreviation Lyn is derived from Lck/Yes novel tyrosine kinase, Lck and Yes also being members of the Src kinase family.

Background References

1. Brian BF 4th et al. The Src-family Kinase Lyn in Immunoreceptor Signaling. Endocrinology. 2021 Oct

2. Fattet L et al. Matrix Rigidity Controls Epithelial-Mesenchymal Plasticity and Tumor Metastasis via a Mechanoresponsive EPHA2/LYN Complex. Dev Cell. 2020 Aug

Subcellular Location

Cell membrane, Nucleus, Cytoplasm, perinuclear region, Golgi apparatus, Membrane.

UNIPROT

SwissProt: P07948 Human

SwissProt: P25911 Mouse

SwissProt: Q07014 Rat

Synonyms

AA407514 antibody

EC 2.7.10.2 antibody

FLJ26625 antibody

Hck 2 antibody

JTK 8 antibody

JTK8 antibody

Lck/Yes related novel protein tyrosine kinase antibody

LYN antibody

LYN proto oncogene, Src family tyrosine kinase antibody

LYN_HUMAN antibody

Expand

Images

  • Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (<a href="/products/HA721991" style="font-weight: bold;text-decoration: underline;">HA721991</a>) at 1/1,000 dilution.<br /><br />Lane 1: Ramos cell lysate (15 µg/Lane)<br />Lane 2: A431 cell lysate (15 µg/Lane)<br />Lane 3: Jurkat cell lysate (15 µg/Lane)<br />Lane 4: THP-1 cell lysate (15 µg/Lane)<br />Lane 5: Raji cell lysate (15 µg/Lane)<br />Lane 6: K-562 cell lysate (15 µg/Lane)<br />Lane 7: U-937 cell lysate (15 µg/Lane)<br />Lane 8: Mouse lung tissue lysate (30 µg/Lane)<br />Lane 9: Rat lung tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 59 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 30 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721991" style="font-weight: bold;text-decoration: underline;">HA721991</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (HA721991) at 1/1,000 dilution.

    Lane 1: Ramos cell lysate (15 µg/Lane)
    Lane 2: A431 cell lysate (15 µg/Lane)
    Lane 3: Jurkat cell lysate (15 µg/Lane)
    Lane 4: THP-1 cell lysate (15 µg/Lane)
    Lane 5: Raji cell lysate (15 µg/Lane)
    Lane 6: K-562 cell lysate (15 µg/Lane)
    Lane 7: U-937 cell lysate (15 µg/Lane)
    Lane 8: Mouse lung tissue lysate (30 µg/Lane)
    Lane 9: Rat lung tissue lysate (30 µg/Lane)

    Predicted band size: 59 kDa
    Observed band size: 55 kDa

    Exposure time: 30 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721991) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (<a href="/products/HA721991" style="font-weight: bold;text-decoration: underline;">HA721991</a>) at 1/2,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si Lyn cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721991" style="font-weight: bold;text-decoration: underline;">HA721991</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (HA721991) at 1/2,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si Lyn cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 55 kDa

    Exposure time: 1 minute; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721991) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of A431 cells labeling Lyn.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721991" style="font-weight: bold;text-decoration: underline;">HA721991</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A431 cells labeling Lyn.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721991, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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