C19orf35 Mouse Monoclonal Antibody [3-B7-G3]
Catalog# M1001-1
C19orf35 Mouse Monoclonal Antibody [3-B7-G3]
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
C19orf35 Mouse Monoclonal Antibody [3-B7-G3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human C19orf35 aa 1-473 / 473.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
Recombinant protein, Hela, MCF-7, PC-3M, human tonsil tissue, human lung tissue, human uterus tissue, human breast tissue.
Conjugation
unconjugated
Clone Number
3-B7-G3
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:50-1:200
Target
Function
C19orf35 and CNRIP1 were specific to diffuse-type compared to the intestinal-type subgroups. Both revealed a similar level of methylation in CNM regardless of the histologic subtype of tumor while the methylation of the tumor tissue varied with the histologic type. C19orf35 was hypermethylated but CNRIP1 was hypomethylated in diffuse-type tumors compared to the CNM while it had an opposite trend in the tumors of the intestinal-type and mixed-type subgroups. Importantly, research suggest the possibility of utilizing these genes as specific markers for diffuse-type GCs. On the other hand, the function of C19orf35 is still unknown while frequent methylation of CNRIP1 has recently been reported in colorectal cancers and adenoma.
Background References
1. Chong Y et al. DNA methylation status of a distinctively different subset of genes is associated with each histologic Lauren classification subtype in early gastric carcinogenesis. Oncol Rep 31(6):2535-44 (2014).
Sequence Similarity
Belongs to the protein kinase superfamily.
Subcellular Location
Actin cytoskeleton.
UNIPROT
Synonyms
C19orf35 antibody
Chromosome 19 open reading frame 35 antibody
CS035_HUMAN antibody
FLJ45778 antibody
Putative uncharacterized protein C19orf35 antibody
Images
-
Western blot analysis of C19orf35 on recombinant protein using anti-C19orf35 antibody at 1/1,000 dilution.
-
ICC staining C19orf35 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining C19orf35 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining C19orf35 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-C19orf35 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-C19orf35 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-C19orf35 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-C19orf35 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"