C12orf51 Mouse Monoclonal Antibody [A2-F5-F10]
Catalog# M1007-22
C12orf51 Mouse Monoclonal Antibody [A2-F5-F10]
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
C12orf51 Mouse Monoclonal Antibody [A2-F5-F10]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human Protein C12orf51 aa 232-516 / 3,996.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Cell, FC
Molecular Weight
Predicted band size: 438/145/98/262 kDa
Positive Control
HepG2, A549, SH-SY5Y, PC-3M, human colon cancer tissue, human kidney tissue, mouse testis tissue.
Conjugation
unconjugated
Clone Number
A2-F5-F10
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50
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IHC-P
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1:50-1:100
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FC
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1:50-1:100
Target
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Background References
1. Eom SY et al.Exome-wide association study identifies genetic polymorphisms of C12orf51, MYL2, and ALDH2 associated with blood lead levels in the general Korean population. Environ Health 16(1):11 (2017).
2. Kim J et al.No Interaction with Alcohol Consumption, but Independent Effect of C12orf51 (HECTD4) on Type 2 Diabetes Mellitus in Korean Adults Aged 40-69 Years: The KoGES_Ansan and Ansung Study. PLoS One 11(2):e0149321 (2016).
Subcellular Location
Membrane. Single-pass membrane protein.
Synonyms
C12orf51 antibody
HECT domain-containing protein 4 antibody
HECTD4 antibody
KIAA0614 antibody
Probable E3 ubiquitin-protein ligase HECTD4 antibody
Images
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Western blot analysis of C12orf51 on SH-SY5Y (1) and A549 (2) using anti-C12orf51 antibody at 1/100 dilution.
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ICC staining C12orf51 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining C12orf51 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"