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DPY30 Mouse Monoclonal Antibody [1-6]

Usd: 350

Specification

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Catalog# M1311-6

DPY30 Mouse Monoclonal Antibody [1-6]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

DPY30 Mouse Monoclonal Antibody [1-6]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within mouse DPY30 aa 1-99 / 99.

Species Reactivity

Human, Mouse

Validated Applications

WB, IF-Cell

Molecular Weight

11kDa

Positive Control

F9

Conjugation

unconjugated

Clone Number

1-6

RRID

AB_3073071

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

Target

Function

As part of the MLL1/MLL complex, involved in the methylation of histone H3 at 'Lys-4', particularly trimethylation. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. May play some role in histone H3 acetylation. In a teratocarcinoma cell, plays a crucial role in retinoic acid-induced differentiation along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci. May also play an indirect or direct role in endosomal transport.

Background References

1. Crystal structure of the C-terminal domain of human DPY-30-like protein: A component of the histone methyltransferase complex. Wang X., Lou Z., Dong X., Yang W., Peng Y., Yin B., Gong Y., Yuan J., Zhou W., Bartlam M., Peng X., Rao Z. J. Mol. Biol. 390:530-537(2009)

2. A role of histone H3 lysine 4 methyltransferase components in endosomal trafficking. Xu Z., Gong Q., Xia B., Groves B., Zimmermann M., Mugler C., Mu D., Matsumoto B., Seaman M., Ma D. J. Cell Biol. 186:343-353(2009)

3. Role for Dpy-30 in ES cell-fate specification by regulation of H3K4 methylation within bivalent domains. Jiang H., Shukla A., Wang X., Chen W.Y., Bernstein B.E., Roeder R.G. Cell 144:513-525(2011)

Sequence Similarity

Belongs to the dpy-30 family.

Subcellular Location

Golgi apparatus, Nucleus

UNIPROT

SwissProt: Q9C005 Human

Synonyms

Dpy-30-like protein antibody

Dpy-30L antibody

DPY30 antibody

DPY30_HUMAN antibody

HDPY-30 antibody

Protein dpy-30 homolog antibody

Saf19 antibody

Images

  • Western blot analysis on F9 cell lysates using anti-DPY30 Mouse mAb.

    Western blot analysis on F9 cell lysates using anti-DPY30 Mouse mAb.

  • ICC staining DPY30 in F9 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). <br />

    ICC staining DPY30 in F9 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

  • ICC staining DPY30 in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). <br />

    ICC staining DPY30 in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"