AKR7A2 Mouse Monoclonal Antibody [C10-D5]
Catalog# M1505-12
AKR7A2 Mouse Monoclonal Antibody [C10-D5]
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WB
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IF-Cell
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IHC-P
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Human
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Zebrafish
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unconjugated
Overview
Product Name
AKR7A2 Mouse Monoclonal Antibody [C10-D5]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human AKR7A2 aa 97-146 / 359.
Species Reactivity
Human, Zebrafish
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
HepG2, human spermatozoa
Conjugation
unconjugated
Clone Number
C10-D5
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IF-Cell
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1:200
Target
Function
Aflatoxin B1 aldehyde reductase member 2 is an enzyme that in humans is encoded by the AKR7A2 gene. It catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate and may have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). AKR7A2 has broad substrate specificity and NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). AKR7A2 can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro) and reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
Background References
1. "Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of aldo-keto reductase 7 family proteins." Kelly V.P., Sherratt P.J., Crouch D.H., Hayes J.D. Biochem. J. 366:847-861(2002)
2. "Synthesis and catabolism of gamma-hydroxybutyrate in SH-SY5Y human neuroblastoma cells: role of the aldo-keto reductase AKR7A2." Lyon R.C., Johnston S.M., Watson D.G., McGarvie G., Ellis E.M. J. Biol. Chem. 282:25986-25992(2007) "Genetic variation of aflatoxin B1 aldehyde reductase genes (AFAR) in human tumour cells." Praml C., Schulz W., Claas A., Mollenhauer J., Poustka A., Ackermann R., Schwab M., Henrich K.-O.Cancer Lett. 272:160-166(2008)
Sequence Similarity
Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.
Tissue Specificity
Detected in brain, liver, small intestine and testis, and at lower levels in heart, prostate, skeletal muscle and spleen. Detected in kidney proximal and distal tubules, endothelial cells lining the Bowman's capsules and some cysts. Detected at low levels in lung and pancreas (at protein level). Widely expressed.
Subcellular Location
Cytoplasm, Golgi apparatus
UNIPROT
Synonyms
AFAR antibody
AFAR1 antibody
AFB1 aldehyde reductase 1 antibody
AFB1 AR1 antibody
AFB1-AR 1 antibody
AFB1AR1 antibody
Aflatoxin aldehyde reductase antibody
Aflatoxin B1 aldehyde reductase member 2 antibody
Aflatoxin beta1 aldehyde reductase antibody
Aiar antibody
ExpandImages
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Western blot analysis of AKR7A2 on human spermatozoa lysate using anti-AKR7A2 antibody at 1/1,000 dilution.
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ICC staining AKR7A2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Western blot analysis of AKR7A2 on hybrid fish (crucian-carp) liver tissue lysate using anti-AKR7A2 antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"