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MAL Mouse Monoclonal Antibody [B5-G3]

Usd: 185

Specification

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Catalog# M1701-6

MAL Mouse Monoclonal Antibody [B5-G3]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

MAL Mouse Monoclonal Antibody [B5-G3]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within mouse MAL aa 97-146 / 153.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 17 kDa

Positive Control

Mouse kidney tissue, mouse lymphatic vessels tissue lysate, rat brain tissue, rat kidney tissue, mouse brain tissue, Jurkat.

Conjugation

unconjugated

Clone Number

B5-G3

RRID

AB_3073173

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:600

  • FC

  • 1:50-1:200

Target

Function

MAL (myelin and lymphocyte protein), also known as T lymphocyte maturation-associated protein, is a nonglycosylated hydrophobic integral membrane protein belonging to the MAL family of proteolipids. MAL is highly enriched in nervous system myelin and in rafts and apical membranes of epithelial cells. It is involved in forming, stabilizing and maintaining glycosphingolipid-enriched membrane microdomains. MAL maintains the myelin sheath and, by controlling the sorting and trafficking of oligodendrocytes, it is involved in central nervous system paranode maintenance. MAL is a component of lipid rafts in myelinating cells. Association with glycosphingolipids may result in protein-lipid microdomain formation in myelin. MAL has been localized to the endoplasmic reticulum of T cells and in compact myelin of cells in the nervous system. MAL is primarily expressed by oligodendrocytes and Schwann cells in the intermediate and late stages of T cell differentiation.

Background References

1. Carmosino M et al. MAL/VIP17, a new player in the regulation of NKCC2 in the kidney. Mol Biol Cell 21:3985-97 (2010).

2. Kamsteeg EJ et al. MAL decreases the internalization of the aquaporin-2 water channel. Proc Natl Acad Sci U S A 104:16696-701 (2007).

Sequence Similarity

Belongs to the MAL family.

Post-translational Modification

Lipoprotein.

Subcellular Location

Membrane.

UNIPROT

SwissProt: P21145 Human

SwissProt: O09198 Mouse

SwissProt: Q64349 Rat

Synonyms

mal antibody

MAL protein gene antibody

Mal T-cell differentiation protein antibody

MAL_HUMAN antibody

MALGENE antibody

MPV17 antibody

Myelin and lymphocyte protein antibody

T-cell differentiation protein MAL antibody

T-lymphocyte maturation-associated protein antibody

VIP17 antibody

Images

  • Western blot analysis of MAL on mouse kidney (1) and mouse lymphatic vessels (2) tissue lysate using anti-MAL antibody at 1/1,000 dilution.

    Western blot analysis of MAL on mouse kidney (1) and mouse lymphatic vessels (2) tissue lysate using anti-MAL antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-MAL antibody (<a href="/products/M1701-6" style="font-weight: bold;text-decoration: underline;">M1701-6</a>) at 1/600 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1701-6" style="font-weight: bold;text-decoration: underline;">M1701-6</a>) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-MAL antibody (M1701-6) at 1/600 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-6) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-MAL antibody (<a href="/products/M1701-6" style="font-weight: bold;text-decoration: underline;">M1701-6</a>) at 1/1000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1701-6" style="font-weight: bold;text-decoration: underline;">M1701-6</a>) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-MAL antibody (M1701-6) at 1/1000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-6) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-MAL antibody (<a href="/products/M1701-6" style="font-weight: bold;text-decoration: underline;">M1701-6</a>) at 1/1000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1701-6" style="font-weight: bold;text-decoration: underline;">M1701-6</a>) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-MAL antibody (M1701-6) at 1/1000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1701-6) at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"