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MALT1 Recombinant Rabbit Monoclonal Antibody [PSH02-31] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750563

MALT1 Recombinant Rabbit Monoclonal Antibody [PSH02-31] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

MALT1 Recombinant Rabbit Monoclonal Antibody [PSH02-31] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human MALT1 aa 1-400 / 824 (Q9UDY8).

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 92 kDa

Positive Control

HCT 116 cell lysate, HeLa cell lysate, Ramos cell lysate, Daudi cell lysate, K-562 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate, human colon tissue, Jurkat cell lysate, HepG2 cell lysate, 786-0 cell lysate, LNCaP cell lysate, PC-3M cell lysate, Mouse lymph node tissue lysate, human colon cancer tissue.

Conjugation

unconjugated

Clone Number

PSH02-31

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:200

Target

Function

Protease that enhances BCL10-induced activation: acts via formation of CBM complexes that channel adaptive and innate immune signaling downstream of CARD domain-containing proteins (CARD9, CARD11 and CARD14) to activate NF-kappa-B and MAP kinase p38 pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Mediates BCL10 cleavage: MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion. Involved in the induction of T helper 17 cells (Th17) differentiation. Cleaves RC3H1 and ZC3H12A in response to T-cell receptor (TCR) stimulation which releases their cooperatively repressed targets to promote Th17 cell differentiation (By similarity). Also mediates cleavage of N4BP1 in T-cells following TCR-mediated activation, leading to N4BP1 inactivation. May also have ubiquitin ligase activity: binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity.

Background References

1. Wang Q, et al. MALT1 regulates Th2 and Th17 differentiation via NF-κB and JNK pathways, as well as correlates with disease activity and treatment outcome in rheumatoid arthritis. Front Immunol. 2022 Jul 28;13:913830.

2. Gu H, et al. Porcine Reproductive and Respiratory Syndrome Virus Adapts Antiviral Innate Immunity via Manipulating MALT1. mBio. 2022 Jun 28;13(3):e0066422.

Subcellular Location

Cytoplasm, perinuclear region, Nucleus

UNIPROT

SwissProt: Q9UDY8 Human

SwissProt: Q2TBA3 Mouse

Entrez Gene: 307366 Rat

Synonyms

Caspase like protein antibody

DKFZp434L132 antibody

IMD12 antibody

MALT 1 antibody

MALT associated translocation antibody

MALT lymphoma associated translocation antibody

MALT lymphoma-associated translocation antibody

Malt1 antibody

MALT1 paracaspase antibody

MALT1_HUMAN antibody

Expand

Images

  • Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (<a href="/products/HA750563" style="font-weight: bold;text-decoration: underline;">HA750563</a>) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate (15 µg/Lane)<br />Lane 2: HeLa cell lysate (15 µg/Lane)<br />Lane 3: Ramos cell lysate (15 µg/Lane)<br />Lane 4: Daudi cell lysate (15 µg/Lane)<br />Lane 5: K-562 cell lysate (15 µg/Lane)<br />Lane 6: Mouse thymus tissue lysate (30 µg/Lane)<br />Lane 7: Rat thymus tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 92 kDa<br />Observed band size: 92 kDa<br /><br />Exposure time: 2 minutes 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750563" style="font-weight: bold;text-decoration: underline;">HA750563</a>) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (HA750563) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.

    Lane 1: HCT 116 cell lysate (15 µg/Lane)
    Lane 2: HeLa cell lysate (15 µg/Lane)
    Lane 3: Ramos cell lysate (15 µg/Lane)
    Lane 4: Daudi cell lysate (15 µg/Lane)
    Lane 5: K-562 cell lysate (15 µg/Lane)
    Lane 6: Mouse thymus tissue lysate (30 µg/Lane)
    Lane 7: Rat thymus tissue lysate (30 µg/Lane)

    Predicted band size: 92 kDa
    Observed band size: 92 kDa

    Exposure time: 2 minutes 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750563) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MALT1 antibody (<a href="/products/HA750563" style="font-weight: bold;text-decoration: underline;">HA750563</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750563" style="font-weight: bold;text-decoration: underline;">HA750563</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MALT1 antibody (HA750563) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750563) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (<a href="/products/HA750563" style="font-weight: bold;text-decoration: underline;">HA750563</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: Ramos cell lysate (20 µg/Lane)<br />Lane 4: K-562 cell lysate (20 µg/Lane)<br />Lane 5: Jurkat cell lysate (20 µg/Lane)<br />Lane 6: HepG2 cell lysate (20 µg/Lane)<br />Lane 7: 786-0 cell lysate (20 µg/Lane)<br />Lane 8: LNCaP cell lysate (20 µg/Lane)<br />Lane 9: PC-3M cell lysate (20 µg/Lane)<br />Lane 10: Mouse lymph node tissue lysate (no heat) (40 µg/Lane)<br />Lane 11: Mouse thymus tissue lysate (40 µg/Lane)<br />Lane 12: Rat thymus tissue lysate (40 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 92 kDa<br /><br />Exposure time: 37 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750563" style="font-weight: bold;text-decoration: underline;">HA750563</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (HA750563) at 1/1,000 dilution.

    Lane 1: HCT 116 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: Ramos cell lysate (20 µg/Lane)
    Lane 4: K-562 cell lysate (20 µg/Lane)
    Lane 5: Jurkat cell lysate (20 µg/Lane)
    Lane 6: HepG2 cell lysate (20 µg/Lane)
    Lane 7: 786-0 cell lysate (20 µg/Lane)
    Lane 8: LNCaP cell lysate (20 µg/Lane)
    Lane 9: PC-3M cell lysate (20 µg/Lane)
    Lane 10: Mouse lymph node tissue lysate (no heat) (40 µg/Lane)
    Lane 11: Mouse thymus tissue lysate (40 µg/Lane)
    Lane 12: Rat thymus tissue lysate (40 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 92 kDa
    Observed band size: 92 kDa

    Exposure time: 37 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750563) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MALT1 antibody (<a href="/products/HA750563" style="font-weight: bold;text-decoration: underline;">HA750563</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750563" style="font-weight: bold;text-decoration: underline;">HA750563</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MALT1 antibody (HA750563) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750563) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (<a href="/products/HA750563" style="font-weight: bold;text-decoration: underline;">HA750563</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa-si NT cell lysate<br />Lane 2: HeLa-si MALT1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 92 kDa<br /><br />Exposure time: 11 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750563" style="font-weight: bold;text-decoration: underline;">HA750563</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of MALT1 on different lysates with Rabbit anti-MALT1 antibody (HA750563) at 1/5,000 dilution.

    Lane 1: HeLa-si NT cell lysate
    Lane 2: HeLa-si MALT1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 92 kDa

    Exposure time: 11 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750563) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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