MCL1 Rabbit Polyclonal Antibody
Catalog# R1510-26
MCL1 Rabbit Polyclonal Antibody
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IF-Cell
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
MCL1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human MCL1 aa 58-130.
Species Reactivity
Human, Mouse
Validated Applications
IF-Cell, IHC-P
Molecular Weight
37 / 29 kDa
Positive Control
HepG2, SK-BR-3, SW480, human colon cancer tissue, human pancreas tissue, mouse prostate tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
B-cell CLL/lymphoma 2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia 1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase 3 (GSK3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors, PI 3-kinase and AKT. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.
Background References
1. Ma L et al. Long non-coding RNA XIST promotes cell growth and invasion through regulating miR-497/MACC1 axis in gastric cancer. Oncotarget 8:4125-4135 (2017).
2. Wang W et al. FXR agonists enhance the sensitivity of biliary tract cancer cells to cisplatin via SHP dependent inhibition of Bcl-xL expression. Oncotarget 7:34617-29 (2016).
Sequence Similarity
Belongs to the Bcl-2 family.
Post-translational Modification
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.; Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.; Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.; Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.
Subcellular Location
Membrane. Cytoplasm. Mitochondrion.
Synonyms
Bcl 2 related protein EAT/mcl1 antibody
Bcl-2-like protein 3 antibody
Bcl-2-related protein EAT/mcl1 antibody
BCL2 related antibody
Bcl2-L-3 antibody
BCL2L3 antibody
EAT antibody
Induced myeloid leukemia cell differentiation protein Mcl 1 antibody
Induced myeloid leukemia cell differentiation protein Mcl-1 antibody
MCL 1 antibody
ExpandImages
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ICC staining MCL1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining MCL1 in SK-BR-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining MCL1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MCL1 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
MCL1 Recombinant Rabbit Monoclonal Antibody [SI16-04]
Application: WB,IF-Tissue,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
MCL1 Recombinant Rabbit Monoclonal Antibody [SI16-04] - BSA and Azide free
Application: WB,IF-Tissue,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated