MCM7 Mouse Monoclonal Antibody [15E1]
Catalog# EM1901-12
MCM7 Mouse Monoclonal Antibody [15E1]
-
WB
-
IHC-P
-
FC
-
Human
-
Mouse
-
Rat
-
Green monkey
-
unconjugated
Overview
Product Name
MCM7 Mouse Monoclonal Antibody [15E1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human MCM7 aa 516-719 / 719.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 81 kDa
Positive Control
HL-60 cell lysate, HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, SW480 cell lysate, LNCaP cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-1 cell lysate, human tonsil tissue, human esophagus tissue, SH-SY5Y.
Conjugation
unconjugated
Clone Number
15E1
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
ICC/IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.
Background References
1. Hallén T et al. MCM7 as a marker of postsurgical progression in non-functioning pituitary adenomas. Eur J Endocrinol. 2021 Apr
2. Ravindran E et al. Homozygous mutation in MCM7 causes autosomal recessive primary microcephaly and intellectual disability. J Med Genet. 2022 May
Sequence Similarity
Belongs to the MCM family.
Post-translational Modification
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Subcellular Location
Nucleus.
Synonyms
CDABP0042 antibody
CDC 47 antibody
CDC47 antibody
CDC47 homolog antibody
Cdc47, S. cerevisiae, homolog of antibody
DNA replication licensing factor MCM7 antibody
Homolog of S. cerevisiae Cdc47 antibody
MCM 2 antibody
MCM 7 antibody
MCM2 antibody
ExpandImages
-
Western blot analysis of MCM7 on different lysates with Mouse anti-MCM7 antibody (EM1901-12) at 1/1,000 dilution.
Lane 1: HL-60 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate
Lane 5: SW480 cell lysate
Lane 6: LNCaP cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: C2C12 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 81 kDa
Observed band size: 81 kDa
Exposure time: 51 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-12, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MCM7 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-12, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Flow cytometric analysis of MCM7 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-12, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
