MDM2 Mouse Monoclonal Antibody [3G2]

Function

p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.

Background References

1. Amaral, J.D., et al. 2012. Live-cell imaging of p53 interactions using a novel Venus-based bimolecular fluorescence complementation system. Biochem. Pharmacol. 5: 745-752.

2. Morgado-Palacin, L., et al. 2012. Ribosomal stress induces L11- and p53-dependent apoptosis in mouse pluripotent stem cells. Cell Cycle 11: 503-510.

3. Sarkar, T.R., et al. 2012. Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells. Mol. Cell. Biol. 32: 320-332.

Subcellular Location

Cytoplasm, Nucleus

UNIPROT

SwissProt: Q00987 Human

Synonyms