MEK6 Recombinant Rabbit Monoclonal Antibody [PSH07-86] - BSA and Azide free
Catalog# HA751194
MEK6 Recombinant Rabbit Monoclonal Antibody [PSH07-86] - BSA and Azide free
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WB
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MEK6 Recombinant Rabbit Monoclonal Antibody [PSH07-86] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human MEK6 aa 1-50.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 37 kDa
Positive Control
HeLa cell lysate, A431 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate.
Conjugation
unconjugated
Clone Number
PSH07-86
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Dual specificity mitogen-activated protein kinase kinase 6 also known as MAP kinase kinase 6 (MAPKK 6) or MAPK/ERK kinase 6 is an enzyme that in humans is encoded by the MAP2K6 gene, on chromosome 17. MAPKK 6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress-induced cell cycle arrest, transcription activation and apoptosis.
Background References
1. Lee S et al. MEK6 Overexpression Exacerbates Fat Accumulation and Inflammatory Cytokines in High-Fat Diet-Induced Obesity. Int J Mol Sci. 2021 Dec
2. Kang S et al. Beiging Modulates Inflammatory Adipogenesis in Salt-Treated and MEK6-Transfected Adipocytes. Cells. 2021 May
Subcellular Location
Nucleus, Cytoplasm, cytoskeleton.
Synonyms
Dual specificity mitogen activated protein kinase kinase 6 antibody
Dual specificity mitogen-activated protein kinase kinase 6 antibody
MAP kinase kinase 6 antibody
MAP2K6 antibody
MAPK/ERK kinase 6 antibody
MAPKK 6 antibody
MAPKK6 antibody
MEK 6 antibody
MEK6 antibody
Mitogen Activated Protein Kinase Kinase 6 antibody
ExpandImages
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This data was developed using HA722917, the same antibody clone in a different buffer formulation.
Western blot analysis of MEK6 on different lysates with Rabbit anti-MEK6 antibody (HA722917) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A431 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: Mouse skeletal muscle tissue lysate (30 µg/Lane)
Lane 7: Rat skeletal muscle tissue lysate (30 µg/Lane)
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: Lane 1-5: 2 minutes 7 seconds; Lane 6-7: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722917) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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