MICA + MICB Rabbit Polyclonal Antibody
Catalog# ER1803-95
MICA + MICB Rabbit Polyclonal Antibody
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WB
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Human
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unconjugated
Overview
Product Name
MICA + MICB Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human MICB aa 1-330 / 383.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, HL-60 cell lysate, HUVEC cell lysate, NIH:OVCAR-3 cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
MHC class I polypeptide-related sequence A/B (MICA/B) is a cell surface glycoprotein encoded by the MICA/B gene located within MHC locus. MICA and MICB are related to MHC class I and has similar domain structure, which is made up of external α1α2α3 domain, transmembrane segment and C-terminal cytoplasmic tail. MICA/B rather functions as a stress-induced ligand for NKG2D receptor. MICA is broadly recognized by NK cells, γδ T cells, and CD8+ αβ T cells which carry NKG2D receptor on their cell surface. As a result of NKG2D-MICA engagement, effector cytolytic responses of T cells and NK cells against epithelial tumor cells expressing MICA are initiated. MICA/B are not associated with β2-microglobulin nor binds peptides as conventional MHC class I molecules do. The heat shock stress pathway is involved in the regulation of MICA expression as transcription of MICA is regulated by promoter heat shock element.
Background References
1. Groh V et al. Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. Science 279:1737-1740 (1998).
2. Steinle A et al. Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family. Immunogenetics 53:279-287 (2001).
Sequence Similarity
Belongs to the MHC class I family. MIC subfamily.
Tissue Specificity
Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue.
Post-translational Modification
Proteolytically cleaved and released from the cell surface of tumor cells.
Subcellular Location
Cell membrane. Cytoplasm.
Synonyms
MHC class I chain-related protein A antibody
MHC class I chain-related protein B antibody
MHC class I polypeptide related sequence A antibody
MHC class I polypeptide related sequence B antibody
MHC class I polypeptide-related sequence A antibody
MIC-A antibody
micA antibody
MICA_HUMAN antibody
MICB antibody
Images
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☑ Relative expression (RE)
Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (ER1803-95) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: HL-60 cell lysate
Lane 4: HUVEC cell lysate
Lane 5: THP-1 cell lysate (negative)
Lane 6: NIH:OVCAR-3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 60 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-95) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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