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MLH1 Mouse Monoclonal Antibody [A10A3]

Usd: 350

Specification

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Catalog# HA601184

MLH1 Mouse Monoclonal Antibody [A10A3]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

MLH1 Mouse Monoclonal Antibody [A10A3]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human MLH1 aa 301-600 / 756.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 85 kDa

Positive Control

A431 cell lysate, HeLa cell lysate, Daudi cell lysate, K-562 cell lysate, HL-60 cell lysate, SW480 cell lysate, human appendix tissue, human tonsil tissue.

Conjugation

unconjugated

Clone Number

A10A3

RRID

AB_3071903

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Background References

1. Yue Q et al. Histone H3K9 Lactylation Confers Temozolomide Resistance in Glioblastoma via LUC7L2-Mediated MLH1 Intron Retention. Adv Sci (Weinh). 2024 May

2. Guan J et al. MLH1 Deficiency-Triggered DNA Hyperexcision by Exonuclease 1 Activates the cGAS-STING Pathway. Cancer Cell. 2021 Jan

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: P40692 Human

Synonyms

COCA 2 antibody

COCA2 antibody

DNA mismatch repair protein Mlh1 antibody

FCC 2 antibody

FCC2 antibody

hMLH 1 antibody

hMLH1 antibody

HNPCC 2 antibody

HNPCC antibody

HNPCC2 antibody

Expand

Images

  • Western blot analysis of MLH1 on different lysates with Mouse anti-MLH1 antibody (<a href="/products/HA601184" style="font-weight: bold;text-decoration: underline;">HA601184</a>) at 1/1,000 dilution.<br /><br />Lane 1: A431 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: Daudi cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: HL-60 cell lysate<br />Lane 6: SW480 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 84.6 kDa<br />Observed band size: 85 kDa<br /><br />Exposure time: 1 minutes 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601184" style="font-weight: bold;text-decoration: underline;">HA601184</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MLH1 on different lysates with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution.

    Lane 1: A431 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: Daudi cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: HL-60 cell lysate
    Lane 6: SW480 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 84.6 kDa
    Observed band size: 85 kDa

    Exposure time: 1 minutes 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601184) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-MLH1 antibody (<a href="/products/HA601184" style="font-weight: bold;text-decoration: underline;">HA601184</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601184" style="font-weight: bold;text-decoration: underline;">HA601184</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601184) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-MLH1 antibody (<a href="/products/HA601184" style="font-weight: bold;text-decoration: underline;">HA601184</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601184" style="font-weight: bold;text-decoration: underline;">HA601184</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601184) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"