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MMP-1 Recombinant Rabbit Monoclonal Antibody [PSH09-56]

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Specification

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Catalog# HA723118

MMP-1 Recombinant Rabbit Monoclonal Antibody [PSH09-56]

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

MMP-1 Recombinant Rabbit Monoclonal Antibody [PSH09-56]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MMP-1 aa 1-469.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 54 kDa

Positive Control

MDA-MB-231 cell lysate, U-87 MG cell lysate, HUVEC cell lysate, A375 cell lysate, human breast cancer tissue, human lung adenocarcinoma tissue.

Conjugation

unconjugated

Clone Number

PSH09-56

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell IP
human
Tested Tested
Tested Tested
Not Recommended
Not Recommended
Not Recommended
mouse
Not Recommended
Not Recommended
 
 
 
rat
Not Recommended
Not Recommended
 
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

Target

Function

Interstitial collagenase, also known as fibroblast collagenase and matrix metalloproteinase-1 (MMP-1), is an enzyme that in humans is encoded by the MMP1 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. MMP-1 was the first vertebrate collagenase both purified to homogeneity as a protein, and cloned as a cDNA. MMP-1 has an estimated molecular mass of 54 kDa. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Specifically, MMP-1 breaks down the interstitial collagens, types I, II, and III.

Background References

1. Natarajan PM et al. Delving into Matrix Metalloproteinase-1 (MMP-1) and its Significance in Periodontal Diseases. J Pharm Bioallied Sci. 2024 Apr

2. Umedera K et al. Design of MMP-1 inhibitors via SAR transfer and experimental validation. Sci Rep. 2022 Dec

Subcellular Location

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: P03956 Human

Synonyms

27 kDa interstitial collagenase antibody

CLG antibody

CLGN antibody

collagenase, fibroblast antibody

collagenase, interstitial antibody

Fibroblast collagenase antibody

Interstitial collagenase antibody

Matrix metallopeptidase 1 (interstitial collagenase) antibody

Matrix metalloprotease 1 antibody

Matrix Metalloproteinase 1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (<a href="/products/HA723118" style="font-weight: bold;text-decoration: underline;">HA723118</a>) at 1/2,000 dilution.<br /><br />Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)<br />Lane 2: U-87 MG cell lysate (20 µg/Lane)<br />Lane 3: HT-29 cell lysate (negative) (20 µg/Lane)<br />Lane 4: HUVEC cell lysate (20 µg/Lane)<br />Lane 5: A375 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 54 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 27 seconds; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723118" style="font-weight: bold;text-decoration: underline;">HA723118</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (HA723118) at 1/2,000 dilution.

    Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
    Lane 2: U-87 MG cell lysate (20 µg/Lane)
    Lane 3: HT-29 cell lysate (negative) (20 µg/Lane)
    Lane 4: HUVEC cell lysate (20 µg/Lane)
    Lane 5: A375 cell lysate (20 µg/Lane)

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

    Exposure time: 27 seconds; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MMP-1 antibody (<a href="/products/HA723118" style="font-weight: bold;text-decoration: underline;">HA723118</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723118" style="font-weight: bold;text-decoration: underline;">HA723118</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MMP-1 antibody (HA723118) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723118) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-MMP-1 antibody (<a href="/products/HA723118" style="font-weight: bold;text-decoration: underline;">HA723118</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723118" style="font-weight: bold;text-decoration: underline;">HA723118</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Rabbit anti-MMP-1 antibody (HA723118) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723118) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"