MMP-11 Recombinant Rabbit Monoclonal Antibody [SN74-08] - BSA and Azide free
Catalog# HA750250
MMP-11 Recombinant Rabbit Monoclonal Antibody [SN74-08] - BSA and Azide free
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MMP-11 Recombinant Rabbit Monoclonal Antibody [SN74-08] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MMP11 aa 439-488 / 488.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P
Target Molecular Weight
Predicted band size: 55 kDa
Positive Control
293T cell lysate, MCF7 cell lysate, mouse kidney tissue lysate, mouse lung tissue lysate, mouse testis tissue lysate, rat testis tissue lysate, SW480, MCF-7, A549, human spleen tissue, human breast carcinoma tissue, mouse spleen tissue.
Conjugation
unconjugated
Clone Number
SN74-08
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including Collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also called stromelysin-1, -2 and -3) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.
Background References
1. Roscilli G et al. Circulating MMP11 and specific antibody immune response in breast and prostate cancer patients. J Transl Med 12:54 (2014).
2. Ghosh MC et al. CXCR4 regulates migration of lung alveolar epithelial cells through activation of Rac1 and matrix metalloproteinase-2. Am J Physiol Lung Cell Mol Physiol 302:L846-56 (2012).
Sequence Similarity
Belongs to the peptidase M10A family.
Tissue Specificity
Specifically expressed in stromal cells of breast carcinomas.
Post-translational Modification
The precursor is cleaved by a furin endopeptidase.
Subcellular Location
Secreted.
Synonyms
Matrix Metalloproteinase 11 antibody
Matrix metalloproteinase-11 antibody
MMP-11 antibody
Mmp11 antibody
MMP11_HUMAN antibody
SL 3 antibody
SL-3 antibody
SL3 antibody
ST3 antibody
STMY3 antibody
ExpandImages
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This data was developed using ET1611-33, the same antibody clone in a different buffer formulation.
Western blot analysis of MMP-11 on different lysates with Rabbit anti-MMP-11 antibody (ET1611-33) at 1/2,000 dilution.
Lane 1: 293T cell lysate (15 µg/Lane)
Lane 2: MCF7 cell lysate (15 µg/Lane)
Lane 3: Mouse kidney tissue lysate (20 µg/Lane)
Lane 4: Mouse lung tissue lysate (20 µg/Lane)
Lane 5: Mouse testis tissue lysate (20 µg/Lane)
Lane 6: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-33) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1611-33, the same antibody clone in a different buffer formulation.
ICC staining of MMP-11 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). -
This data was developed using ET1611-33, the same antibody clone in a different buffer formulation.
ICC staining of MMP-11 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). -
This data was developed using ET1611-33, the same antibody clone in a different buffer formulation.
ICC staining of MMP-11 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-33, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). -
This data was developed using ET1611-33, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MMP-11 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1611-33, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP-11 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1611-33, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-MMP-11 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-33, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1611-33, the same antibody clone in a different buffer formulation.
Western blot analysis of MMP-11 on different lysates with Rabbit anti-MMP-11 antibody (ET1611-33) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: A549 cell lysate
Lane 1: Neuro-2a cell lysate
Lane 1: NIH/3T3 cell lysate
Lane 1: RAW264.7 cell lysate
Lane 1: L-929 cell lysate
Lane 1: PC-12 cell lysate
Lane 1: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-33) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1611-33, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-MMP-11 antibody (ET1611-33) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-33) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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