Skip to content

MMP-9 Recombinant Mouse Monoclonal Antibody [10A1]

Usd: 350

Specification

Bulk Order Quote | Customization Request

Catalog# EM1801-22

MMP-9 Recombinant Mouse Monoclonal Antibody [10A1]

Application

  • ELISA

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

MMP-9 Recombinant Mouse Monoclonal Antibody [10A1]

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein with Human MMP-9 aa 270-400.

Species Reactivity

Human

Validated Applications

ELISA

Positive Control

Recombinant protein with Human MMP-9.

Conjugation

unconjugated

Clone Number

10A1

RRID

AB_3068845

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • ELISA

  • Use at an assay dependent concentration.

Target

Function

Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a matrixin, a class of enzymes that belong to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix. In humans the MMP9 gene encodes for a signal peptide, a propeptide, a catalytic domain with inserted three repeats of fibronectin type II domain followed by a C-terminal hemopexin-like domain.MMP9, along with elastase, appears to be a regulatory factor in neutrophil migration across the basement membrane.MMP9 plays several important functions within neutrophil action, such as degrading extracellular matrix, activation of IL-1β, and cleavage of several chemokines. In a mouse model, MMP9 deficiency resulted in resistance to endotoxin shock, suggesting that MMP9 is important in sepsis.

Background References

1. Park JH et al. Promoting Wound Healing Using Low Molecular Weight Fucoidan in a Full-Thickness Dermal Excision Rat Model. Mar Drugs 15:(4) pii: E112. (2017).

2. Chen X et al. The role of miR-497-5p in myofibroblast differentiation of LR-MSCs and pulmonary fibrogenesis. Sci Rep 7:40958 (2017).

Sequence Similarity

Belongs to the peptidase M10A family.

Tissue Specificity

Detected in neutrophils (at protein level). Produced by normal alveolar macrophages and granulocytes.

Post-translational Modification

Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.; N- and O-glycosylated.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P14780 Human

Synonyms

82 kDa matrix metalloproteinase-9 antibody

92 kDa gelatinase antibody

92 kDa type IV collagenase antibody

CLG 4B antibody

CLG4B antibody

Collagenase Type 4 beta antibody

Collagenase type IV 92 KD antibody

EC 3.4.24.35 antibody

Gelatinase 92 KD antibody

Gelatinase B antibody

Expand

Images

  • Indirect ELISA analysis of MMP-9 was performed by coating wells of a 96-well plate with 50 µL per well of MMP-9 diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of MMP-9 monoclonal antibody serial diluted starting from a concentration of 4µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Indirect ELISA analysis of MMP-9 was performed by coating wells of a 96-well plate with 50 µL per well of MMP-9 diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1%BSA blocking buffer for 1 hour at 37℃, and incubated with 50 µL per well of MMP-9 monoclonal antibody serial diluted starting from a concentration of 4µg/mL for 45 minutes at 37℃. The plate was washed and incubated with 50 µL per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1/80,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

Products with the same target and pathway

metafield image

MMP-9 Recombinant Rabbit Monoclonal Antibody [JA80-73]

Application: WB,IF-Tissue,IHC-P,FC,mIHC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

MMP-9 Mouse Monoclonal Antibody [2G3]

Application: WB,IP,IF,IHC-P

Reactivity: Human

Conjugate: unconjugated