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MYL9 Recombinant Rabbit Monoclonal Antibody [JE34-63]

Usd: 385

Specification

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Catalog# HA721361

MYL9 Recombinant Rabbit Monoclonal Antibody [JE34-63]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

MYL9 Recombinant Rabbit Monoclonal Antibody [JE34-63]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human MYL9 aa 51-100 / 172.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 20 kDa

Positive Control

Hela cell lysate, mouse colon tissue lysate, rat colon tissue lysate, rat uterus tissue lysate, human colon tissue, human skin tissue, mouse heart tissue, rat skeletal muscle tissue, human stomach tissue lysates.

Conjugation

unconjugated

Clone Number

JE34-63

RRID

AB_3072478

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Myosin regulatory light polypeptide 9 is a protein that in humans is encoded by the MYL9 gene. Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.

Background References

1. Iwamura C et al. Elevated Myl9 reflects the Myl9-containing microthrombi in SARS-CoV-2-induced lung exudative vasculitis and predicts COVID-19 severity. Proc Natl Acad Sci U S A. 2022 Aug

2. Matsushita K et al. Clinicopathological significance of MYL9 expression in pancreatic ductal adenocarcinoma. Cancer Rep (Hoboken). 2022 Oct

Subcellular Location

Cytoplasm, cytoskeleton, cell cortex.

UNIPROT

SwissProt: P24844 Human

SwissProt: Q9CQ19 Mouse

SwissProt: Q64122 Rat

Synonyms

20 kDa myosin light chain antibody

Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody

LC20 antibody

MGC3505 antibody

MLC 2 antibody

MLC-2C antibody

MLC2 antibody

MLY 9 antibody

MRLC1 antibody

MYL9 antibody

Expand

Images

  • Western blot analysis of MYL9 on different lysates with Rabbit anti-MYL9 antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hela cell lysate<br />Lane 2: Mouse colon tissue lysate<br />Lane 3: Rat colon tissue lysate<br />Lane 4: Rat uterus tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 20 kDa<br />Observed band size: 19 kDa<br /><br />Exposure time: 2 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MYL9 on different lysates with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution.

    Lane 1: Hela cell lysate
    Lane 2: Mouse colon tissue lysate
    Lane 3: Rat colon tissue lysate
    Lane 4: Rat uterus tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 20 kDa
    Observed band size: 19 kDa

    Exposure time: 2 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721361) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of MYL9 on human stomach tissue lysates with Rabbit anti-MYL9 antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 20 kDa<br />Observed band size: 19 kDa<br /><br />Exposure time: 1 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MYL9 on human stomach tissue lysates with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 20 kDa
    Observed band size: 19 kDa

    Exposure time: 1 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721361) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MYL9 antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721361) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-MYL9 antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721361) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-MYL9 antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721361) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-MYL9 antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721361" style="font-weight: bold;text-decoration: underline;">HA721361</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721361) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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