Monoamine Oxidase A / MAO-A Recombinant Mouse Monoclonal Antibody [5D1-R]
Catalog# HA601319
Monoamine Oxidase A / MAO-A Recombinant Mouse Monoclonal Antibody [5D1-R]
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WB
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IHC-P
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Human
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Mouse
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Rat
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HA610191
不含抗保成分
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unconjugated
Overview
Product Name
Monoamine Oxidase A / MAO-A Recombinant Mouse Monoclonal Antibody [5D1-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human MAO-A aa 222-517 / 527.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 60 kDa
Positive Control
Rat brain tissue lysate, mouse brain tissue lysate, human colon cancer tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
5D1-R
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
This gene is one of two neighboring gene family members that encode mitochondrial enzymes which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. Mutation of this gene results in Brunner syndrome. Has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues.This gene has also been associated with a variety of other psychiatric disorders, including antisocial behavior. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
Background References
1. De Colibus L. et al. Three-dimensional structure of human monoamine oxidase A (MAO A): relation to the structures of rat MAO A and human MAO B. Proc. Natl. Acad. Sci. U.S.A. 102:12684-12689(2005).
2. Sjoeblom T. al. The consensus coding sequences of human breast and colorectal cancers. Science 314:268-274(2006).
Subcellular Location
Mitochondrion outer membrane.
Synonyms
Amine oxidase [flavin containing] A antibody
Amine oxidase [flavin-containing] A antibody
AOFA antibody
AOFA_HUMAN antibody
EC 1.4.3.4 antibody
MAO A antibody
MAO-A antibody
maoA antibody
Monoamine oxidase A antibody
Monoamine oxidase type A antibody
Images
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Western blot analysis of Monoamine Oxidase A / MAO-A on different lysates with Mouse anti-Monoamine Oxidase A / MAO-A antibody (HA601319) at 1/1,000 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 70 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601319) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-Monoamine Oxidase A / MAO-A antibody (HA601319) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601319) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Monoamine Oxidase A / MAO-A antibody (HA601319) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601319) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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