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Myelin Basic Protein Rabbit Polyclonal Antibody

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Catalog# ER1912-82

Myelin Basic Protein Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Tissue

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Myelin Basic Protein Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

KLH conjugated synthetic peptide derived from Gpig MBP: 69-85/167

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue, FC

Molecular Weight

33 kDa

Positive Control

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-2000

  • IHC-P

  • 1:100-500

  • IF-tissue

  • 1:100-500

  • FC

  • 1ug/test: 1:5000-10000

Target

Function

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

Background References

暂无

Subcellular Location

Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.

UNIPROT

P02686

Synonyms

GDB antibody

Golli MBP antibody

Golli MBP

myelin basic protein antibody

Hemopoietic MBP antibody

HMBPR antibody

HUGO antibody

MBP antibody

MBP_CAVPO antibody

MBP_HUMAN antibody

Expand

Images

  • 25 ug total protein per lane of various lysates (see on figure) probed with MBP polyclonal antibody, unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

    25 ug total protein per lane of various lysates (see on figure) probed with MBP polyclonal antibody, unconjugated (ER1912-82) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

    Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

  • Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue, C02-04002) was used to stain the cell nuclei.

    Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MBP Polyclonal Antibody, Unconjugated (ER1912-82) at 1:200 overnight at 4℃. Followed by conjugated Goat Anti-Rabbit IgG antibody (Rose red), DAPI (blue, C02-04002) was used to stain the cell nuclei.

  • Blank control: A549. <br />Primary Antibody (green line): Rabbit Anti-MBP antibody (<a href="/products/ER1912-82" style="font-weight: bold;text-decoration: underline;">ER1912-82</a>) <br />Dilution: 1μg /10^6 cells; <br />Isotype Control Antibody (orange line): Rabbit IgG . <br />Secondary Antibody : Goat anti-rabbit IgG-PE<br />Dilution: 1μg /test. <br />Protocol<br />The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Blank control: A549.
    Primary Antibody (green line): Rabbit Anti-MBP antibody (ER1912-82)
    Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 1μg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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