Myoglobin Rabbit Polyclonal Antibody
Catalog# ER1901-17
Myoglobin Rabbit Polyclonal Antibody
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Myoglobin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human Myoglobin aa 1-140 / 154.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 17 kDa
Positive Control
MCF-7, rat heart muscle tissue, mouse skeletal muscle tissue, mouse heart muscle tissue, SiHa.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:200
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FC
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1:50-1:100
Target
Function
Myoglobin (symbol Mb or MB) is an iron- and oxygen-binding protein found in the cardiac and skeletal muscle tissue of vertebrates in general and in almost all mammals. Myoglobin is distantly related to hemoglobin. Compared to hemoglobin, myoglobin has a higher affinity for oxygen and does not have cooperative binding with oxygen like hemoglobin does. In humans, myoglobin is only found in the bloodstream after muscle injury. High concentrations of myoglobin in muscle cells allow organisms to hold their breath for a longer period of time. Diving mammals such as whales and seals have muscles with particularly high abundance of myoglobin. Myoglobin is found in Type I muscle, Type II A, and Type II B; although many texts consider myoglobin not to be found in smooth muscle, this has proved erroneous: there is also myoglobin in smooth muscle cells. Myoglobin was the first protein to have its three-dimensional structure revealed by X-ray crystallography. This achievement was reported in 1958 by John Kendrew and associates. For this discovery, Kendrew shared the 1962 Nobel Prize in chemistry with Max Perutz. Despite being one of the most studied proteins in biology, its physiological function is not yet conclusively established: mice genetically engineered to lack myoglobin can be viable and fertile, but show many cellular and physiological adaptations to overcome the loss. Through observing these changes in myoglobin-depleted mice, it is hypothesised that myoglobin function relates to increased oxygen transport to muscle, and to oxygen storage; as well, it serves as a scavenger of reactive oxygen species. In humans, myoglobin is encoded by the MB gene. Myoglobin can take the forms oxymyoglobin (MbO2), carboxymyoglobin (MbCO), and metmyoglobin (met-Mb), analogously to hemoglobin taking the forms oxyhemoglobin (HbO2), carboxyhemoglobin (HbCO), and methemoglobin (met-Hb).
Background References
1. Kagen L et al. Serum myoglobin in myocardial infarction: the “staccato phenomenon.” Is acute myocardial infarction in man an intermittent event Am. J. Med. 62:86-92 (1977).
2. Geurts van Kessel A et al. The human myoglobin gene: a third dispersed globin locus in the human genome. Nucleic Acids Res 12:3235-3243 (1984).
Sequence Similarity
Belongs to the globin family.
Subcellular Location
Cytosol. Secreted.
Synonyms
MB antibody
MGC13548 antibody
MYG_HUMAN antibody
Myoglobin antibody
PVALB antibody
Images
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Western blot analysis of Myoglobin on different lysates with Rabbit anti-Myoglobin antibody (ER1901-17) at 1/1,000 dilution.
Lane 1: MCF7 (Human breast cancer cell) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 120 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1901-17, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 17.2 kDa
Observed band size: 17 kDa -
Immunohistochemical analysis of paraffin-embedded rat heart muscle tissue using anti-Myoglobin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-17, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Myoglobin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-17, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse heart muscle tissue using anti-Myoglobin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-17, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Myoglobin was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-17, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Myoglobin Recombinant Rabbit Monoclonal Antibody [SC06-38]
Application: WB,IF-Cell,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Myoglobin Mouse Monoclonal Antibody [A4B7]
Application: WB,IHC-P,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
Myoglobin Recombinant Rabbit Monoclonal Antibody [SC06-38]
Application: WB,IF-Cell,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated