Skip to content

N-Cadherin Rabbit Polyclonal Antibody

Usd: 295

Specification

Bulk Order Quote | Customization Request

Catalog# ER0503

N-Cadherin Rabbit Polyclonal Antibody

Application

  • WB

  • IHC

  • IF

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

N-Cadherin Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide corresponding to Human N-Cadherin aa 391-440 / 906 (H412G).

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC, IF

Molecular Weight

Predicted band size: 100 kDa

Positive Control

Brain,Epithelium,Liver

Conjugation

unconjugated

RRID

AB_3069012

Product Features

Form

Liquid

Concentration

Storage Instructions

-15℃ to -25℃/1 year(Do not lower than -25℃)

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC

  • 1:100-1:300

  • IF

  • 1:200-1:1,000

Target

Function

The cadherins are a large family of cell adhesion molecules involved in calcium-dependent recognition and adhesion events. The cadherin subfamily includes N-, P-, B-, and E- cadherins, and other members. Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. N-Cadherin is commonly found in cancer cells and provides a mechanism for transendothelial migration. When a cancer cell adheres to the endothelial cells of a blood vessel it up-regulates the src kinase pathway, which phosphorylates beta-catenins attached to both N-cadherin (this protein) and E-cadherins.

Background References

1. Liu J et al. Loss of DLG5 promotes breast cancer malignancy by inhibiting the Hippo signaling pathway. Sci Rep 7:42125-42125 (2017).

2. Chen R et al. Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 8:651-661 (2009).

Post-translational Modification

Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).; May be phosphorylated by OBSCN.

Subcellular Location

Cell membrane

UNIPROT

SwissProt: P19022 Human

SwissProt: P15116 Mouse

SwissProt: Q9Z1Y3 Rat

Synonyms

CADH2_HUMAN antibody

Cadherin 2 antibody

Cadherin 2 N cadherin neuronal antibody

Cadherin 2 type 1 antibody

Cadherin 2 type 1 N cadherin neuronal antibody

Cadherin 2, type 1, N-cadherin (neuronal) antibody

Cadherin-2 antibody

Cadherin2 antibody

Calcium dependent adhesion protein neuronal antibody

CD325 antibody

Expand

Images

  • Immunofluorescence analysis of rat-lung tissue. 1,N-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

    Immunofluorescence analysis of rat-lung tissue. 1,N-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

  • Immunofluorescence analysis of rat-kidney tissue. 1,N-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

    Immunofluorescence analysis of rat-kidney tissue. 1,N-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

  • Immunofluorescence analysis of mouse-kidney tissue. 1,N-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

    Immunofluorescence analysis of mouse-kidney tissue. 1,N-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

  • Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,N-Cadherin Polyclonal Antibody was diluted at 1:200(4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

    Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,N-Cadherin Polyclonal Antibody was diluted at 1:200(4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

  • Immunohistochemical analysis of paraffin-embedded Human kidney. 1,N-Cadherin Polyclonal Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

    Immunohistochemical analysis of paraffin-embedded Human kidney. 1,N-Cadherin Polyclonal Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

  • Western Blot analysis of Jurkat cells using N-Cadherin Polyclonal Antibody diluted at 1:1000

    Western Blot analysis of Jurkat cells using N-Cadherin Polyclonal Antibody diluted at 1:1000

  • Western Blot analysis of K562 cells using N-Cadherin Polyclonal Antibody diluted at 1:1000

    Western Blot analysis of K562 cells using N-Cadherin Polyclonal Antibody diluted at 1:1000

  • Western blot analysis of lysates from COLO205 and K562 cells, using CDH2 Antibody. The lane on the right is blocked with the synthesized peptide.

    Western blot analysis of lysates from COLO205 and K562 cells, using CDH2 Antibody. The lane on the right is blocked with the synthesized peptide.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

metafield image

N Cadherin Recombinant Rabbit Monoclonal Antibody [SY02-46]

Application: WB,IHC-P,IHC-Fr,IF-Tissue

Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig

Conjugate: unconjugated

Products with the same target and pathway

metafield image

N-Cadherin Mouse Monoclonal Antibody [A10-4]

Application: WB,IHC-P

Reactivity: Human,Mouse

Conjugate: unconjugated