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N Cadherin Recombinant Antibody [SY02-46] - Mouse IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610298

N Cadherin Recombinant Antibody [SY02-46] - Mouse IgG1 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

N Cadherin Recombinant Antibody [SY02-46] - Mouse IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human N Cadherin aa 161-210 / 906.

Species Reactivity

Human, Mouse, Rat

Validated Applications

IHC-Fr, IHC-P

Target Molecular Weight

Predicted band size: 100 kDa

Positive Control

Human liver tissue, mouse liver tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

SY02-46

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy-terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5.

Background References

1. You A et al. Metformin sensitizes sorafenib to inhibit postoperative recurrence and metastasis of hepatocellular carcinoma in orthotopic mouse models. J Hematol Oncol 9:20 (2016).

2. Fischer KD et al. Vitamin D Supplementation Reduces Induction of Epithelial-Mesenchymal Transition in Allergen Sensitized and Challenged Mice. PLoS One 11:e0149180 (2016).

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P19022 Human

SwissProt: P15116 Mouse

SwissProt: Q9Z1Y3 Rat

Synonyms

CADH2_HUMAN antibody

Cadherin 2 antibody

Cadherin 2 N cadherin neuronal antibody

Cadherin 2 type 1 antibody

Cadherin 2 type 1 N cadherin neuronal antibody

Cadherin 2, type 1, N-cadherin (neuronal) antibody

Cadherin-2 antibody

Cadherin2 antibody

Calcium dependent adhesion protein neuronal antibody

CD325 antibody

Expand

Images

  • This data was developed using <a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: liver<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    This data was developed using HA601464, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Mouse

    Site: liver

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>, the same antibody clone in a different buffer formulation.<br />Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: liver<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    This data was developed using HA601464, the same antibody clone in a different buffer formulation.
    Application: IHC-Fr

    Species: Rat

    Site: liver

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • This data was developed using <a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-N Cadherin antibody (<a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601464, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-N Cadherin antibody (HA601464) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601464) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-N Cadherin antibody (<a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601464, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-N Cadherin antibody (HA601464) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601464) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • This data was developed using <a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>, the same antibody clone in a different buffer formulation.<br />Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-N Cadherin antibody (<a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601464" style="font-weight: bold;text-decoration: underline;">HA601464</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    This data was developed using HA601464, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-N Cadherin antibody (HA601464) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601464) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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