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NAK / TBK1 Mouse Monoclonal Antibody [A8D9]

Usd: 350

Specification

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Catalog# HA601045

NAK / TBK1 Mouse Monoclonal Antibody [A8D9]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

NAK / TBK1 Mouse Monoclonal Antibody [A8D9]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human TBK1 aa 530-729/729.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 84 kDa

Positive Control

K562 cell lysate, NIH/3T3 cell lysate, Hela cell lysate, MCF-7 cell lysate, HepG2 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat stomach tissue lysate.

Conjugation

unconjugated

Clone Number

A8D9

RRID

AB_3071769

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

TBK1 (TANK-binding kinase 1) is an enzyme with kinase activity. Specifically, it is a serine / threonine protein kinase. It is encoded by the TBK1 gene in humans. This kinase is mainly known for its role in innate immunity antiviral response. However, TBK1 also regulates cell proliferation, apoptosis, autophagy, and anti-tumor immunity. Insufficient regulation of TBK1 activity leads to autoimmune, neurodegenerative diseases or tumorigenesis. TBK1 is involved in many signaling pathways and forms a node between them. For this reason, regulation of its involvement in individual signaling pathways is necessary. This is provided by adaptor proteins that interact with the dimerization domain of TBK1 to determine its location and access to substrates. Binding to TANK leads to localization to the perinuclear region and phosphorylation of substrates which is required for subsequent production of type I interferons (IFN-I). In contrast, binding to NAP1 and SINTBAD leads to localization in the cytoplasm and involvement in autophagy. Another adaptor protein that determines the location of TBK1 is TAPE. TAPE targets TBK1 to endolysosomes.

Background References

1. Hu L. et. al. TBK1 Is a Synthetic Lethal Target in Cancer with VHL Loss. Cancer Discov. 2020 Mar

2. Aravamudhan A. et. al. TBK1 regulates YAP/TAZ and fibrogenic fibroblast activation. Am J Physiol Lung Cell Mol Physiol. 2020 May

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q9UHD2 Human

SwissProt: Q9WUN2 Mouse

SwissProt: D4A7D3 Rat

Synonyms

EC 2.7.11.1 antibody

FLJ11330 antibody

FTDALS4 antibody

NAK antibody

NF kappa B activating kinase antibody

NF kB activating kinase antibody

NF-kappa-B-activating kinase antibody

Serine/threonine protein kinase TBK 1 antibody

Serine/threonine protein kinase TBK1 antibody

Serine/threonine-protein kinase TBK1 antibody

Expand

Images

  • Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (<a href="/products/HA601045" style="font-weight: bold;text-decoration: underline;">HA601045</a>) at 1/2,000 dilution.<br /><br />Lane 1: K562 cell lysate<br />Lane 2: NIH/3T3 cell lysate<br />Lane 3: Hela cell lysate<br />Lane 4: MCF-7 cell lysate<br />Lane 5: HepG2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 84 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 2 minutes;<br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601045" style="font-weight: bold;text-decoration: underline;">HA601045</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601045) at 1/2,000 dilution.

    Lane 1: K562 cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: Hela cell lysate
    Lane 4: MCF-7 cell lysate
    Lane 5: HepG2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 84 kDa
    Observed band size: 80 kDa

    Exposure time: 2 minutes;
    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601045) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (<a href="/products/HA601045" style="font-weight: bold;text-decoration: underline;">HA601045</a>) at 1/500 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br />Lane 3: Rat stomach tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 84 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 2 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601045" style="font-weight: bold;text-decoration: underline;">HA601045</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601045) at 1/500 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate
    Lane 3: Rat stomach tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 84 kDa
    Observed band size: 80 kDa

    Exposure time: 2 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601045) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (<a href="/products/HA601045" style="font-weight: bold;text-decoration: underline;">HA601045</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si NAK / TBK1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 84 kDa<br />Observed band size: 80 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601045" style="font-weight: bold;text-decoration: underline;">HA601045</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of NAK / TBK1 on different lysates with Mouse anti-NAK / TBK1 antibody (HA601045) at 1/1,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si NAK / TBK1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 84 kDa
    Observed band size: 80 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601045) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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