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NCAM1 / CD56 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1204-1

NCAM1 / CD56 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

NCAM1 / CD56 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide corresponding to Mouse NCAM1 aa 101-150 / 1,115 mouse.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 95 kDa

Positive Control

SH-SY5Y cell lysate, NCCIT cell lysate, F9 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y, N2A.

Conjugation

unconjugated

RRID

AB_3073196

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50

Target

Function

Neural Cell Adhesion Molecule (NCAM, also the cluster of differentiation CD56) is a homophilic binding glycoprotein expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory. NCAM is a glycoprotein of Immunoglobulin (Ig) superfamily. The three main isoforms of NCAM vary only in their cytoplasmic domain: NCAM-120kDa (GPI anchored), NCAM-140kDa (short cytoplasmic domain) and NCAM-180kDa (long cytoplasmic domain).

Background References

1. "Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56)."Lanier L.L., Chang C., Azuma M., Ruitenberg J.J., Hemperly J.J., Phillips J.H.J. Immunol. 146:4421-4426(1991)

2. "Phosphoproteomic analysis of synaptosomes from human cerebral cortex."DeGiorgis J.A., Jaffe H., Moreira J.E., Carlotti C.G. Jr., Leite J.P., Pant H.C., Dosemeci A.J. Proteome Res. 4:306-315(2005)

Subcellular Location

Cell membrane, secreted

UNIPROT

SwissProt: P13591 Human

SwissProt: P13595 Mouse

SwissProt: P13596 Rat

Synonyms

antigen recognized by monoclonal antibody

5.1H11 antibody

CD56 antibody

Cell adhesion molecule, neural, 1 antibody

MSK39 antibody

N-CAM 140 antibody

N-CAM-1 antibody

NCAM antibody

NCAM-1 antibody

NCAM1 antibody

Expand

Images

  • Western blot analysis of NCAM1 / CD56 on different lysates with Rabbit anti-NCAM1 / CD56 antibody (<a href="/products/R1204-1" style="font-weight: bold;text-decoration: underline;">R1204-1</a>) at 1/1,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 2: NCCIT cell lysate (20 µg/Lane)<br />Lane 3: F9 cell lysate (20 µg/Lane)<br />Lane 4: C6 cell lysate (20 µg/Lane)<br />Lane 5: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 6: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 95 kDa<br />Observed band size: 120-180 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/R1204-1" style="font-weight: bold;text-decoration: underline;">R1204-1</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NCAM1 / CD56 on different lysates with Rabbit anti-NCAM1 / CD56 antibody (R1204-1) at 1/1,000 dilution.

    Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 2: NCCIT cell lysate (20 µg/Lane)
    Lane 3: F9 cell lysate (20 µg/Lane)
    Lane 4: C6 cell lysate (20 µg/Lane)
    Lane 5: Mouse brain tissue lysate (40 µg/Lane)
    Lane 6: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 95 kDa
    Observed band size: 120-180 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1204-1) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling NCAM1 / CD56 with Rabbit anti-NCAM1 / CD56 antibody (<a href="/products/R1204-1" style="font-weight: bold;text-decoration: underline;">R1204-1</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NCAM1 / CD56 antibody (<a href="/products/R1204-1" style="font-weight: bold;text-decoration: underline;">R1204-1</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of SH-SY5Y cells labeling NCAM1 / CD56 with Rabbit anti-NCAM1 / CD56 antibody (R1204-1) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NCAM1 / CD56 antibody (R1204-1) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of N2A cells labeling NCAM1 / CD56 with Rabbit anti-NCAM1 / CD56 antibody (<a href="/products/R1204-1" style="font-weight: bold;text-decoration: underline;">R1204-1</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NCAM1 / CD56 antibody (<a href="/products/R1204-1" style="font-weight: bold;text-decoration: underline;">R1204-1</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of N2A cells labeling NCAM1 / CD56 with Rabbit anti-NCAM1 / CD56 antibody (R1204-1) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NCAM1 / CD56 antibody (R1204-1) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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