NDRG2 Recombinant Rabbit Monoclonal Antibody [PSH25-90]
Catalog# HA724582
NDRG2 Recombinant Rabbit Monoclonal Antibody [PSH25-90]
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WB
-
IF-Cell
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NDRG2 Recombinant Rabbit Monoclonal Antibody [PSH25-90]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human NDRG2 aa 1-371.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, IP
Target Molecular Weight
Predicted band size: 41 kDa
Positive Control
HCT 116 (Human colon cancer cell) cell lysate, COLO205 (Human colon adenocarcinoma cell) cell lysate, LoVo (Human colon cancer cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, T-47D (Human breast ductal carcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, CTLL-2 (Mouse T lymphocyte) cell lysate.
Conjugation
unconjugated
Clone Number
PSH25-90
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:500
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IHC-P
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1:1,000
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IP
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1-2μg/sample
Target
Function
Protein NDRG2 is a protein that in humans is encoded by the NDRG2 gene (NMYC downstream-regulated gene 2). This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma and aggressive meningioma carcinogenesis (via cell proliferation). Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Background References
1. Feng D et al. Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage. Sci Adv. 2022 Sep
2. Jiang T et al. Reprogramming astrocytic NDRG2/NF-kappaB/C3 signaling restores the diabetes-associated cognitive dysfunction. EBioMedicine. 2023 Jul
Subcellular Location
Cytoplasm, perinuclear region, Cell projection, growth cone.
Synonyms
Protein NDRG2
N-myc downstream-regulated gene 2 protein
Protein Syld709613
NDRG2
KIAA1248
SYLD
Images
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☑ Relative expression (RE)
Western blot analysis of NDRG2 on different lysates with Rabbit anti-NDRG2 antibody (HA724582) at 1/5,000 dilution.
Lane 1: HCT 116 (Human colon cancer cell) cell lysate
Lane 2: COLO205 (Human colon adenocarcinoma cell) cell lysate
Lane 3: LoVo (Human colon cancer cell) cell lysate
Lane 4: HT-29 (Human colorectal adenocarcinoma cell) cell lysate
Lane 5: T-47D (Human breast ductal carcinoma cell) cell lysate
Lane 6: MCF7 (Human breast cancer cell) cell lysate
Lane 7: MDA-MB-231 (Human breast cancer cell) cell lysate
Lane 8: CTLL-2 (Mouse T lymphocyte) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1802
Low expression: MDA-MB-231 (PMID: 24636131)
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724582, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 41 kDa
Observed band size: 41 kDa -
Application: Immunocytochemistry (IF-cell)
Species: Mouse
Sample: CTLL-2 (Mouse T lymphocyte)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724582, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724582, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724582, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Immunoprecipitation (IP)
NDRG2 was immunoprecipitated in 0.2 mg T-47D (Human breast ductal carcinoma cell) cell lysate with HA724582 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724582 at 1/5,000 dilution. HRP Conjugated Rabbit IgG kappa light chain Mouse Monoclonal Antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: T-47D cell lysate (input)
Lane 2: HA724582 IP in T-47D cell lysate
Lane 3: Rabbit IgG instead of HA724582 in T-47D cell lysate
Exposure time: 1 minute 34 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724582, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 41 kDa
Observed band size: 41 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
NDRG2 Recombinant Rabbit Monoclonal Antibody [PSH25-90] - BSA and Azide free
Application: WB,IF-Cell,IHC-P,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
