NF-H Mouse Monoclonal Antibody [2G1]
Catalog# RT1424
NF-H Mouse Monoclonal Antibody [2G1]
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WB
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IP
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IF
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NF-H Mouse Monoclonal Antibody [2G1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
A neurofilament NF-H protein isolated from a cytoskeletal preparation from brain tissue homogenate of calf origin.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP, IF, IHC-P
Molecular Weight
200 kDa
Positive Control
rat brain tissue, mouse brain tissue.
Conjugation
unconjugated
Clone Number
2G1
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃
Storage Buffer
1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IP
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1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
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IF
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1:50-1:500
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IHC-P
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1:50-1:500
Target
Function
Neurofilament-H (NF-H), for neurofilament heavy polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease.
Background References
1. Zhang, L., et al. 2011. Concentration-dependent effect of nerve growth factor on cell fate determination of neural progenitors. Stem Cells Dev. 20: 1723-1731.
2. Strong, M.J. 1999. Neurofilament metabolism in sporadic amyotrophic lateral sclerosis. J. Neurol. Sci. 169: 170-177.
3. Nakamura, Y., et al. 1999. Casein kinase II is responsible for phosphorylation of NF-L at Ser-473. FEBS Lett. 455: 83-86.
Subcellular Location
Cytoplasm, membrane
UNIPROT
Synonyms
200 kDa neurofilament protein antibody
CMT2CC antibody
Nefh antibody
Neurofilament heavy polypeptide 200kDa antibody
Neurofilament heavy polypeptide antibody
Neurofilament triplet H protein antibody
NF H antibody
NF-H antibody
NFH antibody
NFH_HUMAN antibody
Images
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Western blot analysis of NF-H expression in rat brain (A) and mouse brain (B) tissue extracts.
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Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing membrane and cytoplasmic staining of Purkinje cells and neuropil staining in granular layer and molecular layer (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing membrane and cytoplasmic staining of Purkinje cells and neuropil staining in granular layer and molecular layer (B).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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