NKG2D Rabbit Polyclonal Antibody
Catalog# HA500626
NKG2D Rabbit Polyclonal Antibody
-
WB
-
Human
-
unconjugated
Overview
Product Name
NKG2D Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within rat NKG2D aa 151-200.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 24 kDa
Positive Control
HCT 116 cell lysate, A549 cell lysate, HepG2 cell lysate, K-562 cell lysate.
Conjugation
unconjugated
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
Target
Function
NKG2D is an activating receptor (transmembrane protein) belonging to the NKG2 family of C-type lectin-like receptors. NKG2D is encoded by KLRK1 (killer cell lectin like receptor K1) gene which is located in the NK-gene complex (NKC) situated on chromosome 6 in mice and chromosome 12 in humans. In mice, it is expressed by NK cells, NK1.1+ T cells, γδ T cells, activated CD8+ αβ T cells and activated macrophages. In humans, it is expressed by NK cells, γδ T cells and CD8+ αβ T cells. NKG2D recognizes induced-self proteins from MIC and RAET1/ULBP families which appear on the surface of stressed, malignant transformed, and infected cells.
Background References
1. Fuertes MB et al. Leveraging NKG2D Ligands in Immuno-Oncology. Front Immunol. 2021 Jul
2. Hartmann JA et al. Evasion of NKG2D-mediated cytotoxic immunity by sarbecoviruses. Cell. 2024 May
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD314 antibody
CD314 antigen antibody
D12S2489E antibody
Killer cell lectin like receptor subfamily K member 1 antibody
Killer cell lectin-like receptor subfamily K member 1 antibody
KLR antibody
KLRC4 KLRK1 readthrough antibody
KLRK1 antibody
NK cell receptor D antibody
NK lectin-like receptor antibody
ExpandImages
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Western blot analysis of NKG2D on different lysates with Rabbit anti-NKG2D antibody (HA500626) at 1/5,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 34 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500626) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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