NLRC3 Rabbit Polyclonal Antibody
Catalog# ER1706-69
NLRC3 Rabbit Polyclonal Antibody
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
NLRC3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human NLRC3 aa 985-1,034 / 1,065.
Species Reactivity
Human, Mouse
Validated Applications
IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 115 kDa
Positive Control
MCF-7, SH-SY5Y, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse colon tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
NLRC3, short for NOD-like receptor family CARD domain containing 3, is an intracellular protein that plays a role in the immune system. It was previously known as nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 (NOD3) and CLR16.2. NLRC3 inhibits the activity of T cells. NLRC3 also inhibits the mTOR signalling pathway to block cellular proliferation.
Background References
1. Conti B J et al. CATERPILLER 16.2 (CLR16.2), a novel NBD/LRR family member that negatively regulates T cell function. J Biol Chem 280:18375-18385 (2005).
2. Schneider M et al. The innate immune sensor NLRC3 attenuates Toll-like receptor signaling via modification of the signaling adaptor TRAF6 and transcription factor NF-kappaB. Nat Immunol 13:823-831 (2012).
Sequence Similarity
Belongs to the NLRP family.
Subcellular Location
Cytoplasm.
Synonyms
CARD15-like antibody
CARD15-like protein antibody
Caterpiller 16.2 antibody
Caterpiller protein 16.2 antibody
CLR16.2 antibody
FLJ00348 antibody
NLR family CARD domain containing 3 antibody
Nlrc3 antibody
NLRC3_HUMAN antibody
NOD-like receptor C3 antibody
ExpandImages
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ICC staining NLRC3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining NLRC3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NLRC3 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"