NPY2R / Y2 receptor Rabbit Polyclonal Antibody
Catalog# ER1901-14
NPY2R / Y2 receptor Rabbit Polyclonal Antibody
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WB
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FC
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Human
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unconjugated
Overview
Product Name
NPY2R / Y2 receptor Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse NPY2R / Y2 receptor aa 327-376 / 381.
Species Reactivity
Human
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
HUVEC cell lysates, MCF7.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5,000
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FC
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1:1,000
Target
Function
Neuropeptide Y (NPY) and its family of five receptors are known to be associated with hypothalamic regulation of feeding behavior, metabolism, and energy homeostasis in both rodents and humans. In particular, knock-out mice for the gene neuropeptide Y 2 receptor, NPY2R, exhibit hyperphagia and excessive weight gain. As a result, research on the gene has focused on genotyping analysis for the identification of single nucleotide polymorphisms (SNPs) in obese human subjects and drug development for manipulation of its expression as a novel therapeutic treatment for obesity. Although primarily expressed in the arcuate nucleus of the hypothalamus, NPY2R may also be found in other tissues including trabecular bone, vascular tissue, and colonic mucosa.
Background References
1. Maron JL et al. Neuropeptide Y2 receptor (NPY2R) expression in saliva predicts feeding immaturity in the premature neonate. PLoS One 7(5):e37870 (2012).
2. Naveilhan P et al. Normal feeding behavior, body weight and leptin response require the neuropeptide Y Y2 receptor. Nat Med 5(10):1188-1193 (1999).
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family.
Tissue Specificity
High levels in amygdala, corpus callosum, hippocampus and subthalamic nucleus. Also detectable in caudate nucleus, hypothalamus and substantia nigra.
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
Neuropeptide Y receptor type 2 antibody
Neuropeptide Y receptor Y2 antibody
NPY-Y2 receptor antibody
NPY2-R antibody
NPY2R antibody
NPY2R_HUMAN antibody
Y2 receptor antibody
Images
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Western blot analysis of NPY2R / Y2 receptor on HUVEC cell lysates with Rabbit anti-NPY2R / Y2 receptor antibody (ER1901-14) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-14) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of MCF7 cells labeling NPY2R / Y2 receptor.
Cells were fixed and permeabilized. Then stained with the primary antibody (ER1901-14, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"