NSUN4 Mouse Monoclonal Antibody [4G3]
Catalog# HA600024
NSUN4 Mouse Monoclonal Antibody [4G3]
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WB
-
IF-Cell
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Human
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unconjugated
Overview
Product Name
NSUN4 Mouse Monoclonal Antibody [4G3]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human NSUN4 aa 1-384.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
MCF7 cell lysate, HEK-293 cell lysate, K-562 cell lysate, HepG2 cell lysate, MCF7.
Conjugation
unconjugated
Clone Number
4G3
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:100
Target
Function
Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4。
Background References
1. Camara Y. et. al. MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome. Cell Metab. 13:527-539(2011).
Subcellular Location
Mitochondrion.
UNIPROT
Synonyms
5 methylcytosine rRNA methyltransferase NSUN4 antibody
EC=2.1.1.- antibody
NOL1/NOP2/Sun domain family member 4 antibody
NOL1/NOP2/Sun domain family, member 4 antibody
NOP2/Sun domain family, member 4 antibody
Nsun4 antibody
NSUN4_HUMAN antibody
Putative methyltransferase NSUN4 antibody
RP4-603I14.2 antibody
Shtap antibody
ExpandImages
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Western blot analysis of NSUN4 on different lysates with Mouse anti-NSUN4 antibody (HA600024) at 1/500 dilution.
Lane 1: MCF7 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: K-562 cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600024) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of MCF7 cells labeling NSUN4 with Mouse anti-NSUN4 antibody (HA600024) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NSUN4 antibody (HA600024) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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