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NUMB Recombinant Rabbit Monoclonal Antibody [PSH25-86]

Usd: 385

Specification

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Catalog# HA724578

NUMB Recombinant Rabbit Monoclonal Antibody [PSH25-86]

Application

  • WB

  • FC(Intra)

Reactivity

  • Human

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

NUMB Recombinant Rabbit Monoclonal Antibody [PSH25-86]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human NUMB aa 301-650.

Species Reactivity

Human, Rat, Green monkey

Validated Applications

WB, FC(Intra)

Molecular Weight

Predicted band size: 71 kDa

Positive Control

U-87 MG (Human glioblastoma cell) cell lysates, SK-OV-3 (Human ovarian cancer cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate.

Conjugation

unconjugated

Clone Number

PSH25-86

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:10,000

  • FC(Intra)

  • 1:1,000

Target

Function

Protein numb homolog is a protein that in humans is encoded by the NUMB gene. The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. The protein Numb is coded for by the gene, NUMB, whose mechanism appears to be evolutionarily conserved. Numb has been extensively studied in both invertebrates and mammals, though its function is best understood in Drosophila. Numb plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. During neurogenesis, Numb localizes to one side of the mother cell such that it is distributed selectively to one daughter cell. This asymmetric division allows a daughter cell containing Numb to acquire a different fate than the other daughter cell.

Background References

1. Kudinov AE et al. Musashi-2 (MSI2) supports TGF- signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016).

2. Zhao C et al. Numb family proteins are essential for cardiac morphogenesis and progenitor differentiation. Development 141:281-95 (2014).

Subcellular Location

Cell membrane, Endosome membrane.

UNIPROT

SwissProt: P49757 Human

SwissProt: Q2LC84 Rat

Synonyms

c14_5527 antibody

C14orf41 antibody

FLJ31314 antibody

h Numb antibody

h-Numb antibody

Numb antibody

Numb homolog (Drosophila) antibody

Numb homolog antibody

Numb protein homolog antibody

NUMB_HUMAN antibody

Expand

Images

  • Western blot analysis of NUMB on U-87 MG (Human glioblastoma cell) cell lysate with Rabbit anti-NUMB antibody (<a href="/products/HA724578" style="font-weight: bold;text-decoration: underline;">HA724578</a>) at 1/10,000 dilution. <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 59 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724578" style="font-weight: bold;text-decoration: underline;">HA724578</a>, 1/10,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 71 kDa <br />Observed band size: 72/71/66/65 kDa

    Western blot analysis of NUMB on U-87 MG (Human glioblastoma cell) cell lysate with Rabbit anti-NUMB antibody (HA724578) at 1/10,000 dilution.

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 59 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724578, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 71 kDa
    Observed band size: 72/71/66/65 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of NUMB on different lysates with Rabbit anti-NUMB antibody (<a href="/products/HA724578" style="font-weight: bold;text-decoration: underline;">HA724578</a>) at 1/10,000 dilution. <br /> <br />Lane 1: SK-OV-3 (Human ovarian cancer cell) cell lysate <br />Lane 2: SK-BR-3 (Human breast cancer cell) cell lysate <br />Lane 3: COS-1 (African green monkey kidney fibroblast) cell lysate <br />Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate <br /> <br />Lysates/proteins at 15 µg/Lane. <br />Exposure time: 3 minutes; ECL: K1801 <br /> <br />Negative control: SK-BR-3 (PMID: 20443831). <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724578" style="font-weight: bold;text-decoration: underline;">HA724578</a>, 1/10,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 71 kDa <br />Observed band size: 72/71/66/65 kDa

    ☑ Relative expression (RE)

    Western blot analysis of NUMB on different lysates with Rabbit anti-NUMB antibody (HA724578) at 1/10,000 dilution.

    Lane 1: SK-OV-3 (Human ovarian cancer cell) cell lysate
    Lane 2: SK-BR-3 (Human breast cancer cell) cell lysate
    Lane 3: COS-1 (African green monkey kidney fibroblast) cell lysate
    Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 3 minutes; ECL: K1801

    Negative control: SK-BR-3 (PMID: 20443831).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724578, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 71 kDa
    Observed band size: 72/71/66/65 kDa

  • Application: Flow Cytometry (Intra)<br /><br />Species: Rat<br />Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.<br />Antibody dilution buffer: 1x PBS.<br /><br />Primary antibody: <a href="/products/HA724578" style="font-weight: bold;text-decoration: underline;">HA724578</a> (1/1,000, Red) compared with Rabbit IgG Isotype Control (<a href="/products/HA722127" style="font-weight: bold;text-decoration: underline;">HA722127</a>, Green), 15 minutes at room temperature.<br />Secondary antibody: Goat Anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 15 minutes at room temperature.

    Application: Flow Cytometry (Intra)

    Species: Rat
    Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated))

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
    Antibody dilution buffer: 1x PBS.

    Primary antibody: HA724578 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
    Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"