Ndufs4 Recombinant Rabbit Monoclonal Antibody [JE40-47]
Catalog# ET7109-09
Ndufs4 Recombinant Rabbit Monoclonal Antibody [JE40-47]
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WB
-
IHC-P
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FC
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Ndufs4 Recombinant Rabbit Monoclonal Antibody [JE40-47]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Ndufs4 aa 1-140 / 175.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC, IP
Target Molecular Weight
Predicted band size: 20 kDa
Positive Control
HCT 116 cell lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Rat heart tissue lysate, Rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
JE40-47
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:1,000
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FC
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1:50-1:100
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IP
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1:10-1:50
Target
Function
Complex 1 (also known as NADH dehydrogenase) of the electron transport chain (respiratory chain) is an enzymatic complex that catalyzes the transfer of electrons from NADH to ubiquinone. Free energy from the reaction is conserved in the transfer of protons into the intermembrane space to create an electrochemical proton gradient, a driving force for ATP synthesis. Complex 1 is a complicated, multi-protein, L-shaped complex composed of at least 45 different subunits and located in the mitochondrial inner membrane. NDUFS4 (NADH dehydrogenase (ubiquinone) Fe-S protein 4), also known as AQDQ or CI-18 (Complex I-18kDa protein), belongs to the Complex I NDUFS4 subunit family. NDUFS4 localizes to the matrix side of the inner membrane of the mitochondrion and functions as an accessory subunit of Complex I. Mutations in the gene encoding NDUFS4 can result in Complex I mitochondrial respiratory chain deficiency. Patients with this deficiency may exhibit cardiomyopathy, myopathy, liver failure and neurological disorders.
Background References
1. van den Heuvel L et al. Demonstration of a new pathogenic mutation in human complex I deficiency: a 5-bp duplication in the nuclear gene encoding the 18-kD (AQDQ) subunit. Am J Hum Genet 62:262-268 (1998).
2. Murray J et al. The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification. J Biol Chem 278:13619-13622 (2003).
Sequence Similarity
Belongs to the complex I NDUFS4 subunit family.
Subcellular Location
Mitochondrion.
Synonyms
AQDQ antibody
CI 18 antibody
CI 18 kDa antibody
CI AQDQ antibody
CI-18 kDa antibody
CI-AQDQ antibody
Complex I 18 kDa antibody
Complex I AQDQ antibody
Complex I-18 kDa antibody
Complex I-AQDQ antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of Ndufs4 on different lysates with Rabbit anti-Ndufs4 antibody (ET7109-09) at 1/2,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Ndufs4 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Ndufs4 on different lysates with Rabbit anti-Ndufs4 antibody (ET7109-09) at 1/5,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Rat heart tissue lysate
Lane 4: Rat kidney tissue lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7109-09, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 20 kDa
Observed band size: 16 kDa -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ndufs4 antibody (ET7109-09) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Ndufs4 antibody (ET7109-09) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Ndufs4 antibody (ET7109-09) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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