Noggin Rabbit Polyclonal Antibody
Catalog# ER1802-30
Noggin Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Noggin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant full length protein of Human Noggin.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 26 kDa
Positive Control
Noggin Recombinant protein, PC-3M, human placenta tissue, human liver tissue, mouse liver tissue, rat liver tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50-1:200
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IHC-P
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1:200
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FC
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1:50-1:100
Target
Function
Genetic differentiation of the vertebrate somite necessitates a balance of inductive signals and antagonists. Noggin is a secreted protein that binds and inactivates members of the transforming growth factor-beta (TGFβ) superfamily of signaling proteins, such as bone morphogenetic proteins-2, 4, 7 (BMP2, BMP4, BMP7). Inhibition of BMP signaling by axially secreted Noggin mediates normal vertebrate skeletogenesis and patterning of the neural tube and somite. Spatially, Noggin may effectively antagonize BMP activity by efficiently diffusing through extracellular matrices, thereby creating morpho-genic gradients. Mice embryos that are homozygous null for Noggin, a lethal genotype, display stubby, continuous limbs with lack of joints in the paws and an array of other developmental defects.
Background References
1. Schwaerzer G K et al. New insights into the molecular mechanism of multiple synostoses syndrome (SYNS): mutation within the GDF5 knuckle epitope causes noggin-resistance. J Bone Miner Res 27:429-442 (2012).
2. Wang J et al. A New Subtype of Multiple-Synostoses Syndrome is Caused by a Mutation in GDF6 that Decreases its Sensitivity to Noggin and Enhances its Potency as a BMP Signal. J Bone Miner Res 31:882-889 (2016).
Sequence Similarity
Belongs to the noggin family.
Subcellular Location
Secreted.
Synonyms
Nog antibody
NOGG_HUMAN antibody
Noggin antibody
SYM 1 antibody
SYM1 antibody
Symphalangism 1 (proximal) antibody
Synostoses (multiple) syndrome 1 antibody
SYNS 1 antibody
SYNS1 antibody
Images
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Western blot analysis of Noggin on Recombinant protein using anti-Noggin antibody at 1/500 dilution.
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ICC staining Noggin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Noggin antibody (ER1802-30) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-30) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Noggin antibody (ER1802-30) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-30) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Noggin antibody (ER1802-30) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-30) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Noggin antibody (ER1802-30) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-30) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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